ISINGENISO
Ubhubhane lwekholera, olubangelwa i-V.cholerae serotype O1, lusaqhubeka lube aisifo esibhubhisayo esibaluleke kakhulu emhlabeni wonke kwabaningi abasathuthukaamazwe.Ngokomtholampilo, ikholera ingase isuke ku-asymptomatic colonization kuyeisifo sohudo esibi kakhulu ngokulahleka okukhulu koketshezi, okuholela ekuphelelweni amanzi emzimbeni, i-electrolyteiziphazamiso, nokufa.V. cholerae O1 imbangela lesi sihudo secretory byukuhlangana kwamathumbu amancane kanye nokukhiqizwa kobuthi obunamandla bekholera,Ngenxa yokubaluleka komtholampilo kanye ne-epidemiological yekholera, ibalulekileukunquma ngokushesha ngangokunokwenzeka ukuthi ngabe umzimba uvela esigulini noma changohudo olunamanzi lubonisa ukuthi une-V.cholera O1.Ukushesha, okulula futhi okuthembekileindlela yokuthola i-V.cholerae O1 iyinani elikhulu kodokotela ekulawulenilesi sifo kanye nezikhulu zezempilo zomphakathi ekuqaliseni izindlela zokulawula.

ISIMISO
I-Vibrio cholerae O1 Antigen Rapid Test Device (Indle) ithola i-Vibrioikholera O1 ngokusebenzisa incazelo ebonakalayo yokuthuthukiswa kombala ngaphakathihlubula.Amasosha omzimba e-Anti-Vibrio cholerae O1 awanyakazi endaweni yokuhlolwaulwelwesi.Ngesikhathi sokuhlolwa, isifanekiso siphendula nge-anti-Vibrio cholerae O1amasosha omzimba ahlanganiswe nezinhlayiya ezinemibala futhi ahlanganiswe ngaphambili kuphedi yesampula yeukuhlolwa.Ingxube ibe isihamba nge-membrane ngesenzo se-capillary futhiihlangana nama-reagents kulwelwesi.Uma kune-Vibrio cholerae O1 eyaneleesibonelweni, ibhande elinemibala lizokwakheka endaweni yokuhlola ye-membrane.Iukuba khona kwaleli bhande elinemibala kubonisa umphumela omuhle, kuyilapho ukungabikho kwawoikhombisa umphumela ongemuhle.Ukubukeka kwebhande elinemibala ekulawuleniisifunda sisebenza njengokulawula kwenqubo, okubonisa ukuthi umthamo ofanele weisifanekiso sengeziwe futhi i-membrane wicking yenzekile.

IZINYATHELO ZOKUZIVIKELA
• Okokusetshenziswa kochwepheshe be-in vitro diagnostic kuphela.
• Ungasebenzisi ngemva kosuku lokuphelelwa yisikhathi olukhonjiswe ephaketheni.Ungasebenzisiukuhlolwa uma isikhwama se-foil sonakalisiwe.Ungaphinde usebenzise ukuhlola.
• Le khithi iqukethe imikhiqizo yezilwane.Ulwazi oluqinisekisiwe lwe-imvelaphi kanye/noma isimo sokuhlanzeka kwezilwane akuqinisekisi ngokupheleleukungabi khona kwama-ejenti we-pathogenic adluliselwayo.Ngakho-ke,itusa ukuthi le mikhiqizo iphathwe njengamandla athelelekayo, futhikusingathwa ngokubheka izinyathelo zokuphepha ezijwayelekile (isb., ungadli noma uhogele).
• Gwema ukungcoliswa ngokwehlukana kwezibonelo ngokusebenzisa isifanekiso esishaisitsha sokuqoqa sesampula ngayinye etholiwe.
• Funda yonke inqubo ngokucophelela ngaphambi kokuhlolwa.
• Ungadli, ungaphuzi noma ubheme kunoma iyiphi indawo lapho kuphathwa khona ama-specimens namakhithi.Bamba zonke izifanekiso njengokungathi ziqukethe izinto ezithathelwanayo.Qaphela okuqinisekisiweIzinyathelo zokuvikela ngokumelene nezingozi ze-microbiological kuyo yonke inqubo kanyelandela izinqubo ezijwayelekile zokulahlwa okufanele kwezibonelo.Gqoka okokuzivikelaizingubo ezinjengamajazi aselabhorethri, amagilavu ​​alahlwayo kanye nokuvikelwa kwamehlo lapho izibonelo zihlolwa.
• Isikhumbuzi se-dilution buffer siqukethe i-sodium azide, engase ihlangane nomthofunoma amapayipi ethusi ukuze enze ama-azide ensimbi okungenzeka aqhume.Lapho ulahlayesikhumbuzi sesikhumbuzi sebhafa noma amasampuli akhishiwe, ahlala ewasha ngamanani amaningiamanzi amaningi ukuvimbela ukunqwabelana kwe-azide.
• Ungashintshisani noma uhlanganise ama-reagents avela ezindaweni ezahlukene.
• Umswakama nezinga lokushisa kungaba nomthelela omubi emiphumeleni.
• Izinto zokuhlola ezisetshenzisiwe kufanele zilahlwe ngokwemithetho yendawo.