Inoveli Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit

Incazelo emfushane:

I-coronavirus yenoveli iyigciwane le-RNA, eliqukethe amaprotheni nama-nucleic acid. Leli gciwane lingena emzimbeni womuntu ophethe (umuntu), lingene kumaseli ngendawo ebophayo ehambelana ne-receptor ACE2, bese liyaziphindaphinda kumaseli aphethe, okwenza amasosha omzimba omuntu aphendule kubahlaseli bakwamanye amazwe futhi akhiqize amasosha omzimba athile. Ngakho-ke, i-vial nucleic acid nama-antigen, nama-antibodies athile abhekene ne-coronavirus yenoveli angasetshenziswa njengama-biomarker athile wokuthola i-coronavirus yenoveli. Ngokuthola i-nucleic acid, ubuchwepheshe be-RT-PCR busetshenziswa kakhulu.

I-Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit yenzelwe ukuthi isetshenziselwe ukuthola ukutholakala kwekhwalithi ye-SARS_CoV-2 virus RNA ekhishwe kuma-nasopharyngeal swabs, oropharyngeal swabs, sputum kanye ne-BALF ezigulini ezihambisana ne-FDA / CE Uhlelo lokukhishwa kwe-IVD namapulatifomu we-PCR aqokiwe abhalwe ngenhla.

Ikhithi yenzelwe ukusetshenziswa ngabasebenzi abaqeqeshiwe elebhu


Imininingwane yomkhiqizo

Amathegi womkhiqizo

Le khithi ye-PCR ebucayi, elungele ukusebenziseka iyatholakala ngefomethi ye-lyophilized (inqubo yokumisa okomisa) yokugcina isikhathi eside. Ikhithi ingahanjiswa futhi igcinwe ekamelweni lokushisa futhi izinze unyaka owodwa. Ishubhu ngayinye ye-premix iqukethe wonke ama-reagents adingekayo ekukhuliseni i-PCR, kufaka phakathi i-Reverse-transcriptase, i-Taq polymerase, ama-primers, ama-probes, nama-substrates e-dNTPs. Idinga kuphela ukwengeza amanzi ayi-13ul afakiwe kanye nethempulethi ekhishwe i-RNA engu-5ul, bese ingaqhutshwa futhi ikhuliswe kuzinsimbi ze-PCR.

Umshini we-qPCR kufanele uhlangabezane nalezi zidingo ezilandelayo:
1. Fit 8 strip PCR tube volume 0.2 ml
Yiba neziteshi ezingaphezu kwesine zokubona:

Isiteshi

Ukuthokozisa (nm)

Ukukhishwa (nm)

Odayi Abalinganiselwe Ngaphambili

1.

470

525

FAM, SYBR Okuluhlaza I

2

523

564

VIC, HEX, TET, JOE

3.

571

621

I-ROX, TEXAS-RED

4

630

670

I-CY5

Amapulatifomu e-PCR:
7500Real-Time PCR System, Biorad CF96, iCycler iQ ™ Real-Time PCR Detection System, iStratagene Mx3000P, Mx3005P

Ubunzima bokuthuthwa kwamaketanga abandayo weRevelnt Reagent yeNoveli Coronavirus nucleic acid
Lapho ama-reagents ejwayelekile wokuthola i-nucleic acid ethuthwa ebangeni elide, (-20 ± 5) storage isitoreji esibandayo sokuhamba kanye nokuhamba kuyadingeka ukuqinisekisa ukuthi i-enzyme esebenza ngama-reagents ihlala isebenza. Ukuze kuqinisekiswe ukuthi izinga lokushisa lifinyelela ezingeni, kudingeka amakhilogremu amaningi eqhwa elomile ebhokisini ngalinye le-nucleic acid test reagent noma ngaphansi kuka-50g, kepha lingahlala izinsuku ezimbili noma ezintathu kuphela. Ngokombono wokusebenza kwemboni, isisindo sangempela sama-reagents akhishwe ngabakhiqizi singaphansi kuka-10% (noma sincane kakhulu kunaleli nani) lesitsha. Isisindo esiningi sivela eqhweni elomile, emaphaketheni eqhwa nasemabhokisini amagwebu, ngakho-ke izindleko zokuhamba ziphezulu ngokweqile.

NgoMashi 2020, i-COVID-19 yaqala ukuqhamuka ngesilinganiso esikhulu phesheya, futhi isidingo seNovel Coronavirus nucleic acid reagent reagent sakhula kakhulu. Ngaphandle kwezindleko eziphezulu zokuthekelisa ama-reagents kuketanga elibandayo, iningi labakhiqizi lisengayemukela ngenxa yenani elikhulu nenzuzo ephezulu.

Kodwa-ke, ngokwenziwa ngcono kwezinqubomgomo zikazwelonke zokuthumela ngaphandle kwemikhiqizo elwa nobhadane, kanye nokwenza ngcono ukulawulwa kukazwelonke ngokugeleza kwabantu kanye nezinsiza kusebenza, kukhona ukunwetshwa nokungaqiniseki ngesikhathi sokuthuthwa kwama-reagents, okwaholela ezinkingeni zomkhiqizo ezivelele ngokuhamba. Isikhathi sokuhamba esandisiwe (isikhathi sokuhamba cishe kwesigamu senyanga sivame kakhulu) kuholela ekuhlulekeni komkhiqizo okuvamile lapho umkhiqizo ufinyelela kwiklayenti. Lokhu kukhathaze kakhulu amabhizinisi ama-reagents e-nucleic acid athumela kwamanye amazwe.

Ubuchwepheshe obenziwe nge-Lyophilized for PCR reagent busize ukuthuthwa kwe-Novel Coronavirus nucleic acid detection reagent emhlabeni jikelele

Ama-reagents e-lyophilized PCR angahanjiswa futhi agcinwe ekamelweni lokushisa, elingagcini nje ukunciphisa izindleko zokuhamba, kepha futhi ligweme izinkinga zekhwalithi ezibangelwa inqubo yokuhamba. Ngakho-ke, i-lyophilizing the reagent iyindlela engcono kakhulu yokuxazulula inkinga yokuthuthwa kwempahla kwamanye amazwe.

I-Lyophilization ifaka ukufaka isisombululo esiqandisini sibe sesimweni esiqinile, bese ubeka phansi futhi uhlukanise umusi wamanzi ngaphansi kwesimo se-vacuum. I-solute eyomile ihlala esitsheni enokwakheka okufanayo nomsebenzi. Uma kuqhathaniswa nama-reagents ajwayelekile oketshezi, ingxenye egcwele egcwele i-Novel Coronavirus nucleic acid detage reagent ekhiqizwa yiLiming Bio inezici ezilandelayo:

Ukuqina okukhulu kokushisa: ikwazi ukwelashwa ngokuma kuma-56 ℃ izinsuku ezingama-60, kanti i-morphology nokusebenza kwe-reagent kuhlala kungashintshi.
Isitoreji esijwayelekile sokushisa nokuhamba: asikho isidingo se-chain ebandayo, asikho isidingo sokugcina emazingeni okushisa aphansi ngaphambi kokuvulwa, khipha ngokuphelele indawo yokugcina ebandayo.
Ukulungele ukusetshenziswa: i-lyophilizing yazo zonke izingxenye, asikho isidingo sokumiswa kwesistimu, igwema ukulahleka kwezinto ezine-viscosity ephezulu njenge-enzyme.
Izinhloso ze-Multiplex kuthubhu eyodwa: ilitshe lokuthola lihlanganisa inoveli ye-coronavirus ORF1ab, isakhi sofuzo se-N, isakhi S sokugwema ukwenziwa kwegciwane. Ukuze kuncishiswe okungelona iqiniso, isakhi sofuzo se-RNase P sisetshenziswa njengokulawulwa kwangaphakathi, ukuze kuhlangatshezwane nesidingo somtholampilo sokulawulwa kwekhwalithi yesampula.


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