I-Rotavirus Antigen Rapid Test
ISINGENISO
I-Rotavirus iyi-ejenti evame kakhulu ebhekene ne-acute gastroenteritis, ikakhulukazi ezinganeni ezincane.Ukutholakala kwayo ngo-1973 kanye nokuhlotshaniswa kwayo ne-infantile gastro-enteritis kwakumelela intuthuko ebaluleke kakhulu ocwaningweni lwe-gastroenteritis engabangelwa ukutheleleka kwebhaktheriya okunamandla.I-Rotavirus isakazwa ngomzila we-oral-fecal kanye nesikhathi sokufukamela izinsuku ezingu-1-3.Nakuba izibonelo eziqoqwe phakathi nosuku lwesibili nolwesihlanu lokugula zilungele ukutholwa kwe-antigen, i-rotavirus ingase itholakale ngenkathi isifo sohudo siqhubeka.I-Rotaviral gastroenteritis ingase ibangele ukufa kwabantu abasengozini njengezinsana, abantu abadala kanye neziguli ezingenawo amandla omzimba.Ezimweni ezipholile, izifo ze-rotavirus zenzeka ikakhulukazi ezinyangeni zasebusika.Kuye kwabikwa izifo eziwumqedazwe kanye nezifo eziwumshayabhuqe ezithinta abantu abayinkulungwane.Njengoba izingane ezilaliswe esibhedlela eziphethwe yi-acute enteric disease, kufika ku-50% wezibonelo ezihlaziyiwe zazine-rotavirus.Amagciwane aziphindaphinda ku-
i-cell nucleus futhi ijwayele ukuba umsingathi wezinhlobo ezithile ezikhiqiza umphumela we-cytopathic (CPE).Ngenxa yokuthi i-rotavirus inzima kakhulu kusiko, akuvamile ukusebenzisa ukuhlukaniswa kwegciwane ekuxilongweni kwezifo.Kunalokho, kuye kwasungulwa izindlela ezihlukahlukene zokubona i-rotavirus endle.
ISIMISO
I-Rotavirus Rapid Test Device (Indle) ithola i-rotavirus ngokutolika okubonakalayo kokuthuthukiswa kombala emgqeni wangaphakathi.Ama-anti-rotavirus amasosha omzimba awanyakazi endaweni yokuhlola yolwelwesi.Ngesikhathi sokuhlolwa, isampula
isabela ngama-anti-rotavirus amasosha omzimba ahlanganiswe ezinhlayiyeni ezinemibala futhi ahlanganiswe ngaphambili kuphedi yesampula yokuhlolwa.Ingxube ibe isihamba nge-membrane ngesenzo se-capillary futhi ixhumana nama-reagents kulwelwesi.Uma ekhona
i-rotavirus eyanele emfanekisweni, ibhande elinemibala lizokwakhiwa endaweni yokuhlola ye-membrane.Ukuba khona kwaleli bhande elinemibala kubonisa umphumela omuhle, kuyilapho ukungabikho kwawo kubonisa umphumela omubi.Ukubonakala kwebhande elinemibala e-
isifunda sokulawula sisebenza njengokulawula kwenqubo, okubonisa ukuthi umthamo ofanele we-specimen wengeziwe futhi i-membrane wicking yenzekile.
IZINTO ZEKHITHI
| Amadivayisi okuhlola apakishwe ngawodwana | Idivayisi ngayinye iqukethe umucu onama-conjugate anemibala nama-reagents asebenzayo ambozwe ngaphambilini ezindaweni ezihambisanayo. |
| I-specimens dilution tube ene-buffer | 0.1 M I-Phosphate buffered saline (PBS) kanye ne-0.02% sodium azide. |
| Amapayipi alahlwayo | Okokuqoqwa kwezibonelo eziwuketshezi |
| Faka iphakheji | Ukuze uthole imiyalelo yokusebenza |
IZINTO EZIDINGEKAYO KODWA AKUNIKEZWE
| Isikhathi | Ukusebenzisa isikhathi |
| I-Centrifuge | Ukwelashwa kwezibonelo ezimweni ezikhethekile |
Izitifiketi
