I-Rotavirus antigen ngokushesha
Ukuqalisa
I-Rotavirus yi-ejenti evame kakhulu ebhekele i-gastroenteritis enkulu, ikakhulukazi ezinganeni ezincane. Ukutholwa kwayo ngo-1973 nokuhlangana kwalo nge-Gastro-enteritis yalo kwakufanekisela intuthuko ebaluleke kakhulu esifundweni se-gastroenteritis ayibangelwa ukutheleleka kwamagciwane acisiwe. I-Rotavirus idluliswa yindlela yomlomo enobuhle enesikhathi sokufakwa kwezinsuku ezi-1-3. Yize ama-specimens aqoqwe ngoSuku lwesibili nowesihlanu lokugula alungele ukutholwa kwe-Antigen, i-rotavirus isengatholakala ngenkathi uhudo luqhubeka. Kungaholela ekufeni kwabantu ngokushona kwabantu engcupheni njengezinsana, iziguli zakudala nezigudlampande. Ezimweni sezulu ezifudumele, ukutheleleka kwama-rotavirus kwenzeka ikakhulu ezinyangeni zobusika. Ama-Endemocs kanye nobhubhane othinta abantu abayinkulungwane kuye kwabikwa. Njengoba kunezingane ezilanjiswe esibhedlela ezihlushwa isifo se-acute Entersic, kuze kufike ku-50% wezinhlobo ezihlaziywe ezazinenzuzo e-rotavirus. Amagciwane aphindaphinda ku
I-Cell Nucleus futhi ithambekele ekubambeni izinhlobo ezikhethekile ezikhiqiza umphumela we-cytopathic ocacile (CPE). Ngoba i-rotavirus inzima kakhulu emasikweni, ayijwayelekile ukusebenzisa ukwahlukaniswa kwaleli gciwane ekuhlolweni kwezifo. Esikhundleni salokho, kwasungulwa amasu ahlukahlukene ukuthola i-rotavirus ezindlebeni.
Isimiso
I-Rotavirus Rapid Vitral Service (FECES) ibona i-rotavirus ngokuhunyushwa okubonakalayo kokuthuthukiswa kombala ku-strip yangaphakathi. Ama-antibodies anti-rotavirus afakwa esifundeni sokuhlolwa kolwelwesi. Ngesikhathi sokuhlola, i-specimen
Iphendula ngama-antibodies alwa nama-anti-rotavirus ahlanganiswe nezinhlayiya ezinemibala futhi adluliselwe ephepheni lesampula lokuhlolwa. Ingxube yabe isisuka ngolwelwesi ngesenzo se-capillary futhi ixhumana nama-reagents ku-membrane. Uma kukhona
Kwanele iRotavirus ku-specimen, iqembu elinemibala lizokha esifundeni sokuhlola se-membrane. Ukuba khona kwale band enemibala kukhombisa umphumela omuhle, kanti ukungabikho kwayo kukhombisa umphumela omubi. Ukubukeka kwebhendi enemibala e
Isifunda sokulawula sisebenza njengesilawuli senqubo, okukhombisa ukuthi kufakwe umthamo ofanele we-specimen futhi wenzeke ulwelwesi lwe-membrane.
Izakhi zekhithi
| Amadivayisi wokuhlola agcwele ngawodwa | Idivaysi ngayinye iqukethe umucu onezimbotshana ezinemibala kanye nokuphendula okusebenzayo kuqala ezifundeni ezihambisanayo. |
| Izinhlobo ze-dilution tube nge-buffer | 0.1 m phosphate buffered saline (PBS) no-0.02% sodium Azide. |
| Ama-Pipete alahlekayo | Ngokuqoqa ama-hlobo aketshezi |
| Faka iphakheji | Ngemiyalo yokusebenza |
Izinto ezidingekayo kodwa azinikeziwe
| Umninisikhathi | Ngokusebenzisa isikhathi |
| Guba | Ukwelashwa kwezinhlobo zezinhlobo ezimweni ezikhethekile |
Ukuqinisekiswa
