Inoveli coronavirus (SARS-Cov-2) I-Multiplex Real-Time Pcr KIT
Le kit ebucayi kakhulu, elungele ukusetshenziswa kwe-PCR KIT iyatholakala ngefomethi ye-Lyophilesed (i-Freeze-yomiswa inqubo) ukuze isitoreji isikhathi eside. Ikhithi ingathuthwa futhi igcinwe emazingeni okushisa asekamelweni futhi iqinile unyaka owodwa. I-tube ngayinye ye-Premix iqukethe yonke i-reagents edingekayo ye-PCR Amplication, kufaka phakathi i-Reverse-Transcriptase, iTaq Polymerase, ama-PROMSTS, ama-DNTPS, kanye nama-DNTPS, nezindawo zokuphumula ze-DNTPS. Idinga kuphela ukwengeza amanzi ancishisiwe we-13Ul kanye ne-run ekhishwe i-rna template, khona-ke ingaqhutshwa futhi yandiswa kuzinsimbi ze-PCR.
Umshini we-QPCR kufanele uhlangabezane nalezi zidingo ezilandelayo:
1. Fit 8 strip PCR TUBE VOLULE 0.2 ML
2. Iba neziteshi ezingaphezu kwesine zokuthola:
| Insungubezi | Ukuthokozela (NM) | Ukuphuma (nm) | Amadayi alinganiselwe ngaphambili |
| 1. | 470 | 525 | Fam, Syrb Green I |
| 2 | 523 | 564 | I-VIC, Hex, Tet, Joe |
| 3. | 571 | 621 | Rox, Texas-Red |
| 4 | 630 | I-670 | Cy5 |
Amapulatifomu we-PCR-:
Uhlelo lwe-7500Real-time PCR, biorad CF96, I-ICYCLEER IQ ™ Real-time PCCR PRECET System, Stratagene MX3000P, MX3005P
Ubunzima bokuhanjiswa kwamaketanga okubandayo kwenoveli coronavirus nucleic acid detection reagent
Lapho ama-reagents ajwayelekile we-nucleic acid athuthelwa ebangeni elide, okungu-(-20 Ukuqinisekisa ukuthi izinga lokushisa lifinyelela ezingeni, amakhilogremu ambalwa eqhwa elomile ayadingeka ebhokisini ngalinye le-realent yokuhlola i-nucleic acid ngisho nangaphansi kuka-50g, kepha ingahlala izinsuku ezimbili noma ezintathu kuphela. Ngombono wokuzijwayeza kwezimboni, isisindo sangempela se-reagents esikhishwe ngabakhiqizi singaphansi kwe-10% (noma ngaphansi kwaleli nani) lesitsha. Iningi lesisindo livela eqhweni elomile, amaphakethe eqhwa namabhokisi egwebu, ngakho-ke izindleko zokuhamba ziphezulu kakhulu.
Ngo-Mashi 2020, iCovilit-19 yaqala ukugqashuka ngezinga elikhulu, futhi isidingo senoveli coronavirus reagent reagent yokutholwa kwe-coronavirus inyuke kakhulu. Ngaphandle kwezindleko eziphakeme zokuthumela ama-reagents ku-chain ebandayo, iningi labakhiqizi lingakwamukela ngenxa yenani elikhulu nenzuzo ephezulu.
Kodwa-ke, ngokuthuthuka kwezinqubomgomo zikazwelonke zokuthekelisa zemikhiqizo yokulwa nobhubhane, kanye nokwenziwa ngcono kokulawulwa kwezwe ngokugeleza kwabantu kanye nezinto, kukhona ukungaqiniseki ngesikhathi sokuhamba se-reagents, okuholele ekutheni kube nezinkinga zomkhiqizo ezivelele ezibangelwa ngokuhamba. Isikhathi sokuhamba esandisiwe (isikhathi sokuhamba esingesigamu senyanga sivame kakhulu) siholela ukwehluleka komkhiqizo njalo lapho umkhiqizo ufinyelela kwiklayenti. Lokhu kukhathazeke kakhulu amabhizinisi athumela ama-reagents we-nucleic acid.
Ubuchwepheshe be-Lyophilesed for PCR Repent basize ukuthutha kwenoveli coronavirus nucleic acid detection reagent emhlabeni jikelele
Ama-reachents we-ppcro alolophilized angahanjiswa futhi agcinwe emazingeni okushisa asekamelweni, angakwazi ukunciphisa izindleko zokuhamba kuphela, kepha futhi agweme izinkinga zekhwalithi ezibangelwa inqubo yokuhamba. Ngakho-ke, ukujoyina i-reagent kuyindlela engcono kakhulu yokuxazulula inkinga yokuhamba kwamanye amazwe.
I-Lyophilization ifaka ukuqalisa isixazululo esimweni esiqinile, bese ifaka futhi ihlukanise umphunga wamanzi ngaphansi kwesimo se-vacuum. Isizungu omisiwe sihlala esitsheni esinokwakheka kanye nomsebenzi ofanayo. Uma kuqhathaniswa ne-reagents evamile ye-liquid, i-coronavirus ephelele ye-coronavirus nucleic acid dection reaglent ekhiqizwa nge-ling bio inezici ezilandelayo:
Ukuqina okuqinile kokushisa okuqinile:Kungenzeka ngokuma kwekwelashwa ngo-56 ℃ ngezinsuku ezingama-60, kanye ne-morphology kanye nokwenza i-realent kuhlala kungashintshiwe.
Ukulondolozwa okujwayelekile kokushisa nokuhamba:Akunasidingo se-couck chain, asikho isidingo sokugcina emazingeni okushisa aphansi ngaphambi kokungasho lutho, ukhulule ngokugcwele isikhala sokugcina esibandayo.
Ukulungele ukusetshenziswa:Ukulingisa kwazo zonke izinto, asikho isidingo sokulungiswa kohlelo, ukugwema ukulahlekelwa kwezakhi nge-viscosity ephezulu efana ne-enzyme.
Amatshe we-Multiplex ku-tube eyodwa:Ithagethi yokutholwa ihlanganisa inoveli coronavirus orf1ab gene, n gene, s gene ukugwema i-virus genevariation. Ukuze unciphise ukungahambi kahle kwamanga, i-rnase p gene isetshenziswa njengokulawulwa kwangaphakathi, ukuze ihlangabezane nesidingo somtholampilo sokulawulwa kwekhwalithi yesampula.
