I-Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR Kit
Le kit ye-PCR ezwela kakhulu, elungele ukusetshenziswa itholakala ngefomethi ye-lyophilized (inqubo yokumisa iqhwa) ukuze igcinwe isikhathi eside.Ikhithi ingathuthwa futhi igcinwe ekamelweni lokushisa futhi izinzile unyaka owodwa.Ishubhu ngayinye ye-premix iqukethe wonke ama-reagents adingekayo ukuze kukhuliswe i-PCR, okuhlanganisa i-Reverse-transcriptase, i-Taq polymerase, ama-primers, ama-probe, nama-dNTPs substrates.Idinga kuphela ukungeza amanzi acwecwe angu-13ul kanye nesifanekiso se-RNA esikhishwe ngu-5ul, bese singasetshenziswa futhi sikhuliswe kumathuluzi e-PCR.
Umshini we-qPCR kufanele uhlangabezane nezidingo ezilandelayo:
1. Fit 8 strip PCR tube volume 0.2 ml
2. Yiba neziteshi zokuthola ezingaphezu kwezine:
| Isiteshi | Injabulo (nm) | Ukukhishwa (nm) | Odayi Abakaliwe Ngaphambili |
| 1. | 470 | 525 | FAM, SYBR Green I |
| 2 | 523 | 564 | VIC, HEX, TET, JOE |
| 3. | 571 | 621 | I-ROX, I-TEXAS-RED |
| 4 | 630 | 670 | CY5 |
PCR-izinkundla:
7500Real-Time PCR System, Biorad CF96, iCycler iQ™ Real-Time PCR System, Stratagene Mx3000P, Mx3005P
Ubunzima bokuhamba ngeketango elibandayo leNovel Coronavirus nucleic acid discovery reagent
Lapho ama-reagents ajwayelekile okuthola i-nucleic acid ethuthwa ebangeni elide, isitoreji (-20±5) ℃ seketango elibandayo kanye nokuhamba kuyadingeka ukuze kuqinisekiswe ukuthi i-bioactive ye-enzyme kuma-reagents ihlala isebenza.Ukuze uqiniseke ukuthi izinga lokushisa lifinyelela izinga, amakhilogremu amaningana eqhwa elomile ayadingeka ebhokisini ngalinye le-nucleic acid test reagent ngisho nangaphansi kuka-50g, kodwa lingahlala izinsuku ezimbili noma ezintathu kuphela.Ngokombono wokusebenza kwemboni, isisindo sangempela sama-reagents akhishwe abakhiqizi singaphansi kuka-10% (noma ngaphansi kakhulu kunaleli xabiso) lesiqukathi.Isisindo esiningi sivela eqhweni elomile, amaphakethe eqhwa namabhokisi egwebu, ngakho-ke izindleko zokuhamba ziphezulu kakhulu.
NgoMashi 2020, i-COVID-19 yaqala ukuqubuka ngezinga elikhulu phesheya, futhi isidingo se-Novel Coronavirus nucleic acid discovery reagent sakhula kakhulu.Naphezu kwezindleko eziphakeme zokuthumela ama-reagents ochungechungeni olubandayo, abakhiqizi abaningi basengayamukela ngenxa yobuningi kanye nenzuzo ephezulu.
Kodwa-ke, ngokuthuthukiswa kwezinqubomgomo zikazwelonke zokuthumela ngaphandle kwemikhiqizo elwa nobhubhane, kanye nokuthuthukiswa kokulawula kukazwelonke phezu kokuhamba kwabantu kanye nempahla, kukhona ukunwetshwa nokungaqiniseki ngesikhathi sokuthuthwa kwama-reagents, okuholele ezinkingeni zemikhiqizo ezivelele ezidalwe. ngezokuthutha.Isikhathi sokuthutha esandisiwe (isikhathi sokuthutha cishe isigamu senyanga sivame kakhulu) siholela ekuhlulekeni komkhiqizo njalo lapho umkhiqizo ufinyelela iklayenti.Lokhu kukhathaze amabhizinisi amaningi okuthekelisa ama-nucleic acid.
Ubuchwepheshe be-Lyophilized be-PCR reagent busize ukuthuthwa kwe-Novel Coronavirus nucleic acid yokuthola i-reagent emhlabeni jikelele.
Ama-reagents e-PCR e-lyophilized angathuthwa futhi agcinwe ekamelweni lokushisa, elingakwazi ukunciphisa izindleko zokuthutha kuphela, kodwa futhi ligweme izinkinga zekhwalithi ezibangelwa inqubo yokuthutha.Ngakho-ke, i-lyophilizing the reagent iyindlela engcono kakhulu yokuxazulula inkinga yokuthutha ngaphandle.
I-Lyophilization ihilela ukuqhwaza isixazululo esimweni esiqinile, bese i-sublimate futhi ihlukanise umhwamuko wamanzi ngaphansi kwesimo se-vacuum.I-solute eyomile isala esitsheni esinokwakheka okufanayo nomsebenzi.Uma kuqhathaniswa nama-reagents avamile oketshezi, isakhi esigcwele se-Lyophilized Novel Coronavirus nucleic acid esitholwa yi-Liming Bio sinezici ezilandelayo:
Ukuzinza kokushisa okuqine ngokwedlulele:ingakwazi ngokwelashwa kokuma ku-56℃ izinsuku ezingu-60, futhi i-morphology nokusebenza kwe-reagent kuhlala kungashintshile.
Ukugcinwa kwezinga lokushisa elijwayelekile nokokuthutha:asikho isidingo seketango elibandayo, asikho isidingo sokugcina ekushiseni okuphansi ngaphambi kokuvula, khulula ngokugcwele indawo yokugcina ebandayo.
Ilungele ukusetshenziswa:i-lyophilizing yazo zonke izingxenye, asikho isidingo sokucushwa kwesistimu, ukugwema ukulahlekelwa izingxenye ezine-viscosity ephezulu njenge-enzyme.
Amathagethi amaningi kushubhu eyodwa:okuhlosiwe okuhlosiwe kuhlanganisa isakhi sofuzo se-coronavirus ye-ORF1ab, isakhi sofuzo esingu-N, isakhi sofuzo sika-S ukugwema ukuguquguquka kwegciwane.Ukuze kwehliswe okungalungile okungamanga, isakhi sofuzo se-RNase P yomuntu sisetshenziswa njengokulawula kwangaphakathi, ukuze kuhlangatshezwane nesidingo somtholampilo sokulawulwa kwekhwalithi okuyisampula.
