I-SARS-Cov-2 Antigen Rapid Test (Ukusetshenziswa Kobungcweti)
Ukusetshenziswa okuhlosiwe
I-ConfSTep® SARS-Cov-2 Antigen Rapid Test iyi-Admigen-2 ye-AssayFor Disect ye-SARS-Cov-2 Virus Nuclein Antiin Antiigen e-Asymptos noma, enezimpawu zokutheleleka nge-Covid-19 ngaphakathi okokuqala kuphambuka ekuqaleni kwezimpawu. I-Assay isetshenziswa njengosizo ekutholakaleni kwe-Covid-19. Kuklanywe ukusetshenziselwa ukuhlolwa kokutheleleka nokuxilongwa kwesisulu esibonakalayo nakubantu abase-asymptomatic.
Ukuqalisa
Ama-coronaviruses angamanoveli angowohlobo lwe-β. I-COVIIL-19 yisifo esibuhlungu sokuphefumula. Abantu ngokuvamile bathambekele. Njengamanje, iziguli ezitheleleke yi-coronavirus yenoveli zingumthombo omkhulu wokutheleleka; Abantu abatheleleke nge-asymptomatic bangaba nomthombo othathelwanayo. Ngokusekelwe ophenyweni lwamanje lokuphenya, isikhathi sokufakwa kokufakwa yizinsuku ezi-1 kuye kwezingu-14, iningi lezinsuku ezi-3 kuye kwezingu-7. Ukubonakaliswa okuyinhloko kufaka umkhuhlane, ukukhathala kanye nokukhwehlela owomile. Ukuxinana kwamakhala, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo kutholakala amacala ambalwa.
Isimiso
I-COSTSTESPEP® SARS-Cov-2 Antigen Test isebenzisa ukuhlolwa kwe-Immunochrogamaygraphic. Ama-antibodies ahlanganisiwe we-latex Ama-antibodies we-SARS-COV-2 ahlanganiswe ne-test zone (T) ne-biotin-BSA i-bond endaweni yokulawula (c) Uma ekhona ngesampula, ama-SARS-Cov-2 ama-antigen azobopha ngama-antibodies ahlanganisiwe akha izinhlayiya. Lezi zinhlayiya zizoqhubeka zihambisane nomugqa kuze kube yilapho zihlolelwa indawo (T) lapho zibanjwa khona yi-SARS-Cov-2 Antib Odies ekhiqiza umugqa obomvu obonakalayo. Uma kungekho-ama-antigens-ama-antigen-ama-2 ngesampula, awukho umugqa obomvu owakhiwa endaweni yokuhlola (T). I-streptavidin conjugate izoqhubeka nokufuduka yedwa ize ithathwe endaweni yokulawula (c) nge-biotin-BSA ihlanganisa umugqa ohlaza okwesibhakabhaka, okubonisa ukuba semthethweni kwalesi sivivinyo.
Izakhi zekhithi
| Amadivayisi we-Foil Pool afakwe uphawu angama-25 ahlanganisiwe | Idivayisi ngayinye iqukethe umucu onezimbotshana ezinemibala kanye ne-reagents esebenzayo ngaphambi kokusatshalaliswa ehambelana nalo izifunda. |
| Amashubhu we-Extraction angama-25 ane-pre-egcwele Dilution Buffer | 0.1 m phosphate buffered saline (PBS) no-0.02% sodium azide. |
| Amaphakethe angama-25 we-swab | Ngeqoqo le-specimen. |
| 1 WorkStation | Beka ukubamba ama-buffer vials namashubhu. |
| 1 Iphakheji Faka | Ukufundiswa ukusebenza. |
Izakhi zekhithi
| Umninisikhathi | Ngokusetshenziswa kwesikhathi. |
| Noma yimiphi imishini yokuvikela yomuntu siqu edingekayo |
IZINYATHELO ZOKUZIVIKELA
• Le khithi ingeyokusetshenziswa kokuxilongwa kwe-vitro kuphela.
• Le khithi yenzelwe ukusetshenziswa kwezokwelapha kuphela.
• Funda imiyalo ngokucophelela ngaphambi kokwenza isivivinyo.
• Lo mkhiqizo awunazo izinsiza zomthombo wabantu.
• Musa ukusebenzisa okuqukethwe kwe-Kit ngemuva kosuku lokuphelelwa yisikhathi.
• phatha zonke izinhlobo zezinhlobo njengokutheleleka.
• Landela inqubo yelebhu ejwayelekile kanye nemihlahlandlela ye-byoafety yokuphatha nokulahlwa kwezinto ezingaba khona zomthakathi. Lapho inqubo ye-Assay iqediwe, ilahla amashegu ngemuva kokuwafaka ngokuzenzakalela ku-121 ℃ okungenani imizuzu engama-20. Ngenye indlela, bangalashwa ngo-0.5% sodium hypochlorite amahora amane ngaphambi kokulahlwa.
• Musa ukwenza i-Pipette reagent ngomlomo futhi akukho ukubhema noma ukudla ngenkathi wenza izincazelo.
• Gqoka amagilavu phakathi nenqubo yonke.
• Kuncoma ukusebenzisa idivaysi yesistimu ye-bio ye-bio yokutholwa ngokushesha kwe-SARS-Cov-2 Antigen (ikati # 500210) ukuvikela opharetha kanye nemvelo.
Ukugcinwa nokuqina
Izikhwama ezivalekile ekhitthini lokuhlola zingagcinwa phakathi kuka-2-30 ℃ isikhathi sempilo yeshalofu njengoba kukhonjisiwe esikhwameni.
Ukuqoqwa kweqoqo nesitoreji
Isampula ye-Nasal Swab:
• Faka i-swab eyodwa ibe emakhaleni elilodwa lesiguli. Ithiphu ye-Swab kufanele ifakwe ku-2,5 cm (1 inch) kusuka emaphethelweni ekhala. Roll i-Swab izikhathi ezi-5 eceleni kwe-mucosa ngaphakathi kwenkantolo yokuqinisekisa ukuthi zombili ama-mucus namaseli ayaqoqwa.
• Sebenzisa i-swab efanayo, phinda le nqubo yenye i-Nosstril ukuthi iqinisekise ukuthi isampula eyanele liqoqwa kusuka kuwo wonke ama-nasal emithanjeni.
Sebenzisa i-swab enikezwe ekhishini, ezinye iziteshi ezingathinta kabi ukusebenza kokuhlola, ukusetshenziswa kufanele ziqinisekise i-swab yazo ngaphambi kokuyisebenzisa. Kunconywe ukuthi ama-specimentens acutshungulwe ngokushesha okukhulu ngemuva kokuqoqwa. Izinhlobo ze-specimenters zingabanjwa esitsheni kuze kube yihora eli-1 ekushiseni kwegumbi (15 ° C kuya ku-30 ° C), noma kuze kufike emahoreni angama-24 lapho isiqandisiwe (2 ° C kuye ku-8 ° C) Ngaphambi kokucutshungulwa.
Indlela yokwenza izinto
Letha amadivayisi wokuhlola, ama-specimentens, ama-buffer kanye / noma alawule ekushiseni kwegumbi (15-30 ° C) ngaphambi kokusebenzisa.
• Ngokugcwaliswa kwangaphambi kokugcwaliswa, susa uphawu kusuka ku-vial equkethe i-liquutd.
• Beka i-specimen swab ku-tube. Hlanganisa isixazululo ngokuqinile ngokujikeleza i-swab ngamandla ngokumelene nohlangothi lwe-tube okungenani izikhathi eziyi-15 (ngenkathi kufakwa ngaphakathi). Imiphumela emihle itholakala lapho i-specimen ixubene ngamandla kwikhambi.
• Vumela i-swab ukuthi icwile ekukhipheni i-buffer ukukhishwa umzuzu owodwa ngaphambi kwesinyathelo esilandelayo
• Cwilisa uketshezi oluningi ngangokunokwenzeka kusuka ku-swab ngokucindezela uhlangothi lwe-tube ye-extraction eguquguqukayo njengoba kususwa i-swab. Okungenani okungu-1/2 kwesixazululo sesampula yesampula kumele kuhlale ku-tube yokufuduka okune-capillary okwanele. Faka i-cap ku-tube ekhishwe.
• Lahla i-swab esitsheni esisheshayo se-biohazarduous.
• Mboza i-cap.
• Hlanganisa isixazululo ngokunciphisa i-specimen ngamandla ngokumelene nohlangothi lwe-tube okungenani izikhathi eziyishumi
(ngenkathi kufakwa ngaphakathi). Imiphumela emihle itholakala lapho i-specimen ixubene nesixazululo. Vumela i-specimen ukuthi ingene ku-dilution buffer umzuzu owodwa ngaphambi kwesinyathelo esilandelayo.
• Izinhlobo ezikhishwe zingagcina ekushiseni kwegumbi imizuzu engama-30 ngaphandle kokuthinta umphumela wokuhlolwa.
• Susa idivaysi yokuhlola esikhwameni sayo esivalekile, bese uyibeka endaweni ehlanzekile, yezinga. Faka ilebuli insiza ngesineke noma sokulawula ukukhonjwa. Ukuthola umphumela omuhle kakhulu, i-assay kufanele yenziwe kungakapheli imizuzu engama-30.
• Faka amaconsi ama-3 (cishe ama-100 μl) wesampula ekhishwe kusuka kushubhu lokukhishwa kwisampula eliyindilinga kahle kudivayisi yokuhlola.
• Gwema ukudonsa ama-bubble air esampula, futhi ungalahli noma yisiphi isixazululo ewindini lokubheka. Njengoba ukuhlolwa kuqala ukusebenza, uzobona umbala uhamba ngaphesheya kwe-membrane.
• Lindela ama-band (s) anemibala ukuze avele. Umphumela kufanele ufundwe ngokubonwa ngemizuzu engu-15. Ungahumushi umphumela ngemuva kwemizuzu engama-30.
Lahla amashubhu asetshenzisiwe asetshenzisiwe namadivayisi wokuhlola esitsheni esifanele semfucuza elungele i-biohazardious.
Bhala umyalezo wakho lapha bese usithumela kithi
