Ukuhlolwa kweProcalcitonin
UKUSETSHENZISWA OKUHLOSIWE
Isinyathelo Esiqinile®Ukuhlolwa kwe-Procalcitonin ukuhlola okusheshayo kwe-immune-chromatographic kokutholwa kwe-semi-quantitative ye-Procalcitonin ku-serum yomuntu noma ku-plasma.Isetshenziselwa ukuxilonga nokulawula ukwelashwa kokutheleleka okunzima, okubangelwa amagciwane kanye ne-sepsis.
ISINGENISO
I-Procalcitonin(PCT) iphrotheni encane ehlanganisa izinsalela ze-amino acid ze-116 ezinesisindo se-molecular cishe i-13 kDa eyachazwa okokuqala ngu-Moullec et al.ngo-1984. I-PCT ikhiqizwa ngokuvamile kuma-C-cell of the thyroid glands.Ku-1993, izinga eliphakeme le-PCT ezigulini ezinokutheleleka kwesistimu yemvelaphi yebhaktheriya labikwa futhi i-PCT manje ibhekwa njengophawu oluyinhloko lwezinkinga ezihambisana nokuvuvukala kwesistimu kanye ne-sepsis.Inani lokuxilonga le-PCT libalulekile ngenxa yokuhlobana okuseduze phakathi kokuhlushwa kwe-PCT nobukhulu bokuvuvukala.Kwaboniswa ukuthi i-PCT "yokuvuvukala" ayikhiqizwa kuma-C-cell.Amaseli omsuka we-neuroendocrine cishe awumthombo we-PCT ngesikhathi sokuvuvukala.
ISIMISO
Isinyathelo Esiqinile®I-Procalcitonin Rapid Test ithola i-Procalcitonin ngokuhumusha okubonakalayo kokuthuthukiswa kombala emgqeni wangaphakathi.I-Procalcitonin monoclonal antibody ayinyakazi endaweni yokuhlola yolwelwesi.Ngesikhathi sokuhlolwa, isifanekiso sibhekana nama-anti-Procalcitonin amasosha omzimba e-monoclonal ahlanganiswe nezinhlayiya ezinemibala futhi ahlanganiswe ngaphambili kuphedi ye-conjugate yokuhlola.Ingxube ibe isihamba nge-membrane ngesenzo se-capillary futhi ixhumana nama-reagents kulwelwesi.Uma kune-Procalcitonin eyanele kusifanekiso, ibhande elinemibala lizokwakheka endaweni yokuhlola yolwelwesi.Ukuba khona kwaleli bhande elinemibala kubonisa umphumela omuhle, kuyilapho ukungabikho kwawo kubonisa umphumela omubi.Ukubonakala kwebhande elinemibala endaweni yokulawula kusebenza njengokulawula kwenqubo, okubonisa ukuthi umthamo ofanele we-specimen wengeziwe futhi i-membrane wicking yenzekile.Ukuthuthukiswa kombala okuhlukile endaweni yomugqa wokuhlola(T) kubonisa umphumela omuhle kuyilapho inani le-Procalcitonin lingacutshungulwa ngokomthamo ngokuqhathanisa ukushuba komugqa wokuhlola nokuqina komugqa wereferensi ekhadini lokuhumusha.Ukungabi bikho komugqa onombala endaweni yomugqa wokuhlola(T)
iphakamisa umphumela ongemuhle.
IZINYATHELO ZOKUZIVIKELA
Le kit ingeyokusetshenziswa kokuxilonga i-IN VITRO kuphela.
■ Le khithi isetshenziswa NGOKWENGEZIWE kuphela.
■ Funda imiyalelo ngokucophelela ngaphambi kokwenza isivivinyo.
■ Lo mkhiqizo awunazo izinto eziwumthombo womuntu.
■ Ungasebenzisi okuqukethwe kwekhithi ngemva kosuku lokuphelelwa yisikhathi.
■ Bamba zonke izifanekiso njengalezi ezingase zibe nezithathelanayo.
■ Landela inqubo evamile Yelebhu kanye nemihlahlandlela ye-biosafety yokuphatha nokulahlwa kwezinto ezingaba yingozi.Uma inqubo yokuhlola isiqediwe, lahla izifanekiso ngemva kokuzilungisa ngokuzenzakalelayo ku-121℃ okungenani imizuzu engu-20.Kungenjalo, zingelashwa nge-0.5% ye-Sodium Hypochlorite amahora ngaphambi kokulahlwa.
■ Ungasebenzisi i-pipette reagent ngomlomo futhi ungabhemi noma ungadli ngenkathi uhlola.
■ Gqoka amagilavu phakathi nayo yonke inqubo.