Ukusetshenziswa okuhlosiwe
I-grontstep^®Ukuhlolwa kwe-ProCarcalcitonin kuyinto esheshayo-chromatographic assay yokutholwa kwe-semi-quaty ye-procalcitonin ku-serum yomuntu noma i-plasma. Isetshenziselwa ukuxilonga nokulawula ukwelashwa okunzima, ukutheleleka ngegciwane kanye ne-sepsis.

Ukuqalisa
I-ProCalcitonin (PCT) iprotheni encane enezinsathwa ezingama-116 Amino acid ngesisindo samangqamuzana esingama-13 kda aqala ukuchazwa nge-moullec et al. Ngo-1984. I-PCT ikhiqizwa ngokujwayelekile kumaseli e-C-cell of the Glands. Ngo-1993, izinga eliphakeme le-PCT ezigulini elinokutheleleka kwesistimu yemvelaphi yegciwane kubikwa futhi kubhekwa ukuthi umaki oyinhloko wokuphazamiseka okuhambisana nokuvuvukala okuhlelekile kanye ne-sepsis. Inani lokuxilonga le-PCT libalulekile ngenxa yokuhlangana okusondele phakathi kokuhlushwa kwe-PCT kanye nobunzima bokuvuvukala. Kuboniswe ukuthi i- "Felammatory" PCT ayikhiqizwanga kumaseli e-C. Amaseli we-Neuroendocric avela emthonjeni we-PCT ngesikhathi sokuvuvukala.

Isimiso
I-grontstep^®I-ProCALCITONIN RASD TEV ithola i-ProCapcitonin ngokuhunyushwa kokubukwa kokuthuthukiswa kombala ku-strip yangaphakathi. I-ProCalcitonin Monoclonal antibody ingekho emthethweni esifundeni sokuhlolwa kwe-membrane. Ngesikhathi sokuhlola, izimbongo ziphendula ngama-antibodies anti-anti-anti-antibodies ahlanganiswe nezinhlayiya ezinemibala futhi adluliselwe ku-conjugate pad yesivivinyo. Ingxube yabe isisuka ngolwelwesi ngesenzo se-capillary futhi ixhumana nama-reagents ku-membrane. Uma kune-procalcitonin eyanele e-specimen, ibhendi enemibala izokwakha esifundeni sokuhlolwa kolwelwesi. Ukuba khona kwale band enemibala kukhombisa umphumela omuhle, kanti ukungabikho kwayo kukhombisa umphumela omubi. Ukuvela kwebhendi enemibala esifundeni sokulawula kusebenza njengokulawulwa kwenqubo, okubonisa ukuthi kufakwe umthamo ofanele we-specimen futhi kwenzeke ulwelwesi lwe-membrane. Ukuthuthukiswa kombala okuhlukile esifundeni se-Test Line (T) kukhombisa umphumela omuhle kanti inani le-ProCalcitonin lingahlolwa ngobuningi ngokuqhathanisa ukuqina komugqa wokuhlola ekhadini lokuhumusha. Ukungabikho komugqa onemibala esifundeni sethayi (T)
kuphakamisa umphumela omubi.

IZINYATHELO ZOKUZIVIKELA
Le khithi ingeyokusetshenziswa kokuxilongwa kwe-vitro kuphela.
■ Le khithi yokusetshenziswa kochwepheshe kuphela.
■ Funda imiyalo ngokucophelela ngaphambi kokwenza isivivinyo.
■ Lo mkhiqizo awunazo izinsiza zomthombo wabantu.
■ Ungasebenzisi okuqukethwe kit ngemuva kosuku lokuphelelwa yisikhathi.
■ Phatha zonke izinhlobo zezinto ezingase zitheleleke.
■ Landela inqubo ejwayelekile yelebhu kanye nemihlahlandlela ye-biosafety yokuphatha nokulahlwa kwezinto ezingaba khona zomfazi. Lapho inqubo ye-Assay iqediwe, ilahla ama-specimens ngemuva kokuwafaka ngokuzenzakalela ku-121 ℃ okungenani imizuzu engama-20. Ngenye indlela, bangalashwa ngo-0.5% sodium hypochlorite amahora amaningi ngaphambi kokulahlwa.
■ Musa ukwenza i-Pipette reagent ngomlomo futhi akukho ukubhema noma ukudla ngenkathi imihlandlela yokufaka.
■ Gqoka amagilavu ​​phakathi nenqubo yonke.