Ukuhlolwa kwe-HSV 12 Antigen
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ISINGENISO
I-HSV iyimvilophi, igciwane eliqukethe i-DNA ngokuma ngendlela efana nelinyeamalungu ohlobo lwe-Herpesviridae.Izinhlobo ezimbili ezihlukene ze-antigenicallyeyaziwayo, uhlobo olukhethiwe 1 nohlobo 2.
I-HSV yohlobo 1 no-2 ivamise ukubandakanyeka ezifweni ezingaphandle komlomoumgodi, isikhumba, iso kanye nezitho zangasese, Ukutheleleka kwemizwa ephakathiuhlelo (meningoencephalitis) kanye nokutheleleka okunzima okujwayelekile kwengane esanda kuzalwaisiguli esingenawo amandla okuzivikela omzimba nawo ayabonakala, nakuba engavamile.Ngemva kweukutheleleka okuyinhloko sekuxazululiwe, igciwane lingase libe khona ngendlela efihlekile ekwethukeniizicubu, lapho zingase ziphinde zivele khona, ngaphansi kwezimo ezithile, ukuze kubangele aukubuya kwezimpawu.
Isethulo somtholampilo sakudala se-herpes yangasese siqala ngokusabalalaama-macules nama-papule amaningi abuhlungu, abe esevuthwa abe amaqoqo acacile,ama-vesicles nama-pustules agcwele uketshezi.Ama-vesicles ayaphuka futhi enze izilonda.Isikhumbaizilonda kuqweqwe, kanti izilonda kulwelwesi lwamafinyila ziyaphola ngaphandle koqweqwe.Kukwabesifazane, izilonda zenzeka endaweni ye-introitus, labia, perineum, noma endaweni ye-perianal.Madodangokuvamile kuvame ukuvela izilonda kushaft penial noma inhlaka.Isiguli ngokuvamile siyakhulai-adenopathy yesifo sikashukela.Ukutheleleka kwe-Perianal nakho kujwayelekile ku-MSM.I-Pharyngitis ingakhula ngokuchayeka ngomlomo.
Ucwaningo lwe-Serology luphakamisa ukuthi abantu abayizigidi ezingu-50 e-United States banezitho zangaseseUkutheleleka kwe-HSV.EYurophu, i-HSV-2 itholakala ku-8-15% wabantu jikelele.KuE-Afrika, amazinga okubhebhetheka angama-40-50% kwabaneminyaka engama-20 ubudala.I-HSV ihamba phambiliimbangela yezilonda ezithweni zangasese.Ukutheleleka kwe-HSV-2 okungenani kuphinda kabili ingozi yocansiUkuthola i-human immunodeficiency virus (HIV) futhi kuyandaukudluliswa.
Kuze kube muva nje, ukuhlukaniswa kwegciwane kusiko lweseli nokunqunywa kohlobo lwe-HSVnge-fluorescent staining bekulokhu okuyisisekelo sokuhlolwa kwe-herpes eziguliniukwethula izilonda zesitho sangasese.Ngaphandle kokuhlolwa kwe-PCR kwe-HSV DNAikhonjiswe ukuthi izwela kakhulu kunesiko legciwane futhi inokucaciswa kwayoidlula ama-99.9%.Kodwa lezi zindlela ekusebenzeni komtholampilo okwamanje zilinganiselwe,ngoba izindleko zokuhlolwa kanye nemfuneko yabanolwazi, abaqeqeshiweabasebenzi bezobuchwepheshe ukwenza ukuhlola bakhawule ukusetshenziswa kwabo.
Kukhona futhi ukuhlolwa kwegazi okutholakala ngokwentengiso okusetshenziselwa ukuthola UhloboAmasosha omzimba athile e-HSV, kodwa lokhu kuhlola kwe-serological akukwazi ukubona okuyinhlokoukutheleleka ngakho zingasetshenziswa kuphela ukukhipha izifo eziphindelelayo.Lokhu kuhlolwa kwe-antigen yenoveli kungahlukanisa ezinye izifo zezilonda ezithweni zangasese nezitho zangasesei-herpes, njenge-syphilis ne-chancroid, ukusiza ukuxilongwa kusenesikhathi kanye nokwelashwaukutheleleka nge-HSV.
ISIMISO
I-HSV antigen Rapid Test Device yakhelwe ukuthola i-HSV antigenngokuhumusha okubonakalayo kokuthuthukiswa kombala kumugqa wangaphakathi.II-membrane yayinganyakazi nge-anti Herpes simplex virus monoclonal antibody on
isifunda sokuhlola.Ngesikhathi sokuhlolwa, isifanekiso sivunyelwe ukuthi sisabele ngombalai-monoclonal anti-HSV antibody colored partticals conjugates, efakwe ngaphambiliiphedi yesampula yokuhlolwa.Ingxube ibe isihamba kulwelwesi nge-capillary
isenzo, futhi ixhumana nama-reagents kulwelwesi.Ukube bekune-HSV eyaneleama-antigen kuma-specimens, ibhande elinemibala lizokwakheka endaweni yokuhlola ye-membrane.Ukuba khona kwaleli bhande elinemibala kubonisa umphumela omuhle, kuyilapho ukungabikho kwawo kubonisa
umphumela omubi.Ukubukeka kwebhande elinemibala endaweni yokulawula kusebenza njenge-aukulawulwa kwenqubo.Lokhu kubonisa ukuthi umthamo ofanele wesifanekiso sengeziwefuthi ulwelwesi lwe-membrane lwenzekile.
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