Ukusetshenziswa okuhlosiwe
I-grontstep^®I-ProCALCITONIN Test iyi-immune-chromatographic esheshayoassay kokutholwa okulinganiselwe kwe-procalcitonin ku-serum yomuntu nomaI-PLASMA. Isetshenziselwa ukuxilonga nokulawula ukwelashwa okunzima,ukutheleleka ngegciwane kanye ne-sepsis.

Ukuqalisa
I-ProCALCITONIN (PCT) iprotheni encane equkethe izinsalela ezingama-116 Amino acidngesisindo samangqamuzana esingama-13 KDA aqanjwa okokuqalange-moullec et al. ngo-1984.I-PCT ikhiqizwa ngokujwayelekile kumaseli we-C-ama-gland ama-thyroid. Ngo-1993,Izinga eliphakeme le-PCT ezigulini ngokutheleleka kwesistimu kwemvelaphi yegciwanekubikwa ukuthi i-PCT manje isithathwa njengengoma enkulu yokuphazamisekaehambisana nokuvuvukala okuhlelekile kanye ne-sepsis. Inani lokuxilonga leI-PCT ibalulekile ngenxa yokuhlangana okusondele phakathi kokuhlushwa kwe-PCT kanyeubukhulu bokuvuvukala. Kwaboniswa ukuthi "ukuvuvukala" i-PCT akunjalokukhiqizwe kuma-C-cell. Amaseli we-neuroendocrine avela emthonjeniye-PCT ngesikhathi sokuvuvukala.

Isimiso
I-grontstep^®I-ProCALCITONIN RASD TEV ithola i-ProCapcitonin ngokubukaUkuhunyushwa kokuthuthukiswa kombala ku-strip yangaphakathi. I-ProCALCITONINI-Monoclonal antibody ingenasisekelo esifundeni sokuhlolwa kwe-membrane. NgesikhathiUkuhlola, okucatshangelwe ngama-monoclonal anti-anti-antibodieskuhlanganiswe nezinhlayiya ezinemibala futhi zivezwe ku-conjugate pad yesivivinyo.Ingxube isuka ku-membrane ngesenzo se-capillary futhiuxhumana nama-reagents ku-membrane. Uma kune-plalcitonin eyanele ngaphakathiI-specimen, ibhendi enemibala izokwakha esifundeni sokuhlola sikalwelwesi. Le khasiUkuba khona kwale band enemibala kukhombisa umphumela omuhle, ngenkathi ukungabikho kwayokukhombisa umphumela omubi. Ukubukeka kwebhendi enemibala ekwatsheliniIsifunda sisebenza njengesilawuli senqubo, okubonisa ukuthi umthamo ofanele weI-specimen ingeziwe futhi i-fick wick yenzekile.Ukuthuthukiswa kombala okuhlukile esifundeni somugqa wokuhlola (t) kukhombisa umphumela omuhlekanti inani le-procalcitonin lingahlolwa ngobuningi ngoUkuqhathanisa ukuqina komugqa wokuhlola kumasethirembo wereferensi kuikhadi lokuhumusha. Ukungabikho komugqa onemibala esifundeni sethayi (T)kuphakamisa umphumela omubi.

IZINYATHELO ZOKUZIVIKELA
Le khithi ingeyokusetshenziswa kokuxilongwa kwe-vitro kuphela.
■ Funda imiyalo ngokucophelela ngaphambi kokwenza isivivinyo.
■ Lo mkhiqizo awunazo izinsiza zomthombo wabantu.
■ Ungasebenzisi okuqukethwe kit ngemuva kosuku lokuphelelwa yisikhathi.
■ Phatha zonke izinhlobo zezinto ezingase zitheleleke.
■ Landela inqubo ejwayelekile yelebhu kanye nemihlahlandlela ye-biosafety yokusingatha kanyeukulahlwa kwezinto ezingenzeka ezingokomthakathi. Lapho inqubo ye-assay ikhonaQedela, ulahla ama-specimens ngemuva kokuzivocavotela ngokuzenzakalela ku-121 ℃ okungenani20 imiz. Ngenye indlela, bangalashwa ngo-0.5% sodium hypochloriteamahora amaningi ngaphambi kokulahlwa.
■ Musa ukwenza i-Pipette reagent ngomlomo futhi akukho ukubhema noma ukudla ngenkathi imihlandlela yokufaka.
■ Gqoka amagilavu ​​phakathi nenqubo yonke.