UKUSETSHENZISWA OKUHLOSIWE
Isinyathelo Esiqinile^®I-Procalcitonin Test iyi-immune-chromatographic esheshayoisivivinyo sokutholwa kwe-semi-quantitative ye-Procalcitonin ku-serum yomuntu nomaiplasma.Isetshenziselwa ukuxilonga nokulawula ukwelashwa kwezifo ezinzima,ukutheleleka kwebhaktheriya kanye ne-sepsis.

ISINGENISO
I-Procalcitonin(PCT) iphrotheni encane ehlanganisa izinsalela ze-amino acid eziyi-116.enesisindo samangqamuzana esicishe sibe ngu-13 kDa esachazwa okokuqalanguMoullec et al.ngo-1984.I-PCT ikhiqizwa ngokuvamile kuma-C-cell wezindlala zegilo.Ngo-1993, i-izinga eliphakeme le-PCT ezigulini ezinokutheleleka kwesistimu yemvelaphi yebhaktheriyakwabikwa futhi i-PCT manje isithathwa njengophawu oluyinhloko lwezinkingaehambisana nokuvuvukala kwesistimu kanye ne-sepsis.Inani lokuxilonga leI-PCT ibalulekile ngenxa yokuhlobana okuseduze phakathi kokuhlushwa kwe-PCT kanyeubulukhuni bokuvuvukala.Kwaboniswa ukuthi i-PCT "yokuvuvukala" ayikhoakhiqizwa kuma-C-cell.Amaseli omsuka we-neuroendocrine cishe awumthomboye-PCT ngesikhathi sokuvuvukala.

ISIMISO
Isinyathelo Esiqinile^®I-Procalcitonin Rapid Test ithola i-Procalcitonin ngokubonakalayoukuchazwa kokuthuthukiswa kombala emgqeni wangaphakathi.I-ProcalcitoninI-monoclonal antibody ayinyakazi endaweni yokuhlola yolwelwesi.PhakathiLapho kuhlolwa, isampula sisabela ngamasosha omzimba e-monoclonal anti-Procalcitoninkuhlanganiswe nezinhlayiya ezinemibala futhi kuhlanganiswe ngaphambili kuphedi ye-conjugate yokuhlolwa.Ingxube ibe isihamba nge-membrane ngesenzo se-capillary futhiihlangana nama-reagents kulwelwesi.Uma kukhona iProcalcitonin eyanele kuisampula, ibhande elinemibala lizokwakheka endaweni yokuhlola ye-membrane.Iukuba khona kwaleli bhande elinemibala kubonisa umphumela omuhle, kuyilapho ukungabikho kwawoikhombisa umphumela ongemuhle.Ukubukeka kwebhande elinemibala ekulawuleniisifunda sisebenza njengokulawula kwenqubo, okubonisa ukuthi umthamo ofanele weisifanekiso sengeziwe futhi i-membrane wicking yenzekile.Ukuthuthukiswa kombala okuhlukile endaweni yomugqa wokuhlola(T) kubonisa umphumela omuhlekuyilapho inani le-Procalcitonin lingacutshungulwa ngokomthamoukuqhathaniswa kokuqina komugqa wokuhlola nokuqina komugqa wereferensi kuikhadi lokuhumusha.Ukungabi bikho komugqa onombala endaweni yomugqa wokuhlola(T)iphakamisa umphumela ongemuhle.

IZINYATHELO ZOKUZIVIKELA
Le kit ingeyokusetshenziswa kokuxilonga i-IN VITRO kuphela.
■ Funda imiyalelo ngokucophelela ngaphambi kokwenza isivivinyo.
■ Lo mkhiqizo awunazo izinto eziwumthombo womuntu.
■ Ungasebenzisi okuqukethwe kwekhithi ngemva kosuku lokuphelelwa yisikhathi.
■ Bamba zonke izifanekiso njengalezi ezingase zibe nezithathelanayo.
■ Landela inqubo evamile Yelebhu kanye nemihlahlandlela ye-biosafety yokuphatha kanyeukulahlwa kwezinto ezingaba yingozi.Lapho inqubo yokuhlolaqedela, lahla izifanekiso ngemva kokuzibopha ngokuzenzakalelayo ku-121℃ okungenani20 imiz.Ngaphandle kwalokho, zingelashwa nge-0.5% ye-Sodium Hypochloriteamahora ngaphambi kokulahlwa.
■ Ungasebenzisi i-pipette reagent ngomlomo futhi ungabhemi noma ungadli ngenkathi uhlola.
■ Gqoka amagilavu ​​phakathi nayo yonke inqubo.