Ukusetshenziswa okuhlosiwe
I-grontstep^®I-CryptoCoccal Antigen Rapid Test Idivaysi iyisimo esisheshayo se-chromatographic assay sokutholwa kwe-capsular polysaccharideama-antigens we-cryptococcus izinhlobo eziyinkimbinkimbi (ama-cryptococcus neoformans kanyeI-CryptoCoccus Gattii) ku-serum, i-plasma, igazi lonke kanye ne-cerebral spinal fluid(CSF). I-Assay iyinhlangano esetshenziswayo ye-laboratory esetshenziswayo engasiza kuUkuxilongwa kwe-cryptococcosis.

Ukuqalisa
I-CryptoCoccosis ibangelwa zombili izinhlobo zezinhlobo ze-CryptoCoccus(Ama-cryptococcus neoformans kanye ne-cryptococcus gattii). Abantu abanokukhubazekaUkungavikeleki kwamaseli kusengozini enkulu yokutheleleka. I-CryptoCoccosis ingenyekwezifo ezinamathuba ezivame kakhulu ezigulini ze-AIDS. Ukutholwa kweI-CryptoCoccal Antigen eSerum ne-CSF isetshenziswe kabanzi kakhuluukuzwela okuphezulu nokucaciswa.

Isimiso
I-grontstep^®I-CryptoCoccal Antigen Rapige Test Test yenzelweThola izinhlaka ze-cryptococcus eziyinkimbinkimbi ngokuhunyushwa okubonakalayo kombalaukuthuthukiswa emgqeni wangaphakathi. Ulwelwelwesi lwalungasebenzi kahle nge-antiI-CryptoCoccal Monoclonal antibody esifundeni sokuhlola. Ngesikhathi sokuhlolwa, i-specimenuvunyelwe ukusabela nge-monoclonal anti-cryptococcal antibods enemibalaama-conjugates, avezwa esigabeni sesihlanganisi sokuhlolwa. Ingxube ngaleso sikhathiihamba ngolwelwesi ngesenzo se-capillary, futhi sisebenzisane nama-reagents ku-ulwelwesi. Ukube bekukhona ama-antigens anele ama-cryptococcal ama-antigens, anemibalaIbhendi izokwakha esifundeni sokuhlola sikalwelwesi. Ukuba khona kwaleli band elinemibalaKubonisa umphumela omuhle, kanti ukungabikho kwayo kukhombisa umphumela omubi. UkubonakalaIbhendi enemibala eSifundeni Sokulawula isebenza njengolawulo lwenqubo. Lokhu kukhombisaukuthi ivolumu efanelekile ye-specimen ingeziwe futhi i-membrane wicking inayokwenzeka.

IZINYATHELO ZOKUZIVIKELA
■ Leli khithi lenzelwe ukusetshenziswa kokuxilongwa kwe-vitro kuphela.
■ Le khithi yokusetshenziswa kochwepheshe kuphela.
■ Funda imiyalo ngokucophelela ngaphambi kokwenza isivivinyo.
■ Lo mkhiqizo awunazo izinsiza zomthombo wabantu.
■ Ungasebenzisi okuqukethwe kit ngemuva kosuku lokuphelelwa yisikhathi.
■ Phatha zonke izinhlobo zezinto ezingase zitheleleke.
■ Landela inqubo ejwayelekile yelebhu kanye nemihlahlandlela ye-biosafety yokusingatha kanyeukulahlwa kwezinto ezingenzeka ezingokomthakathi. Lapho inqubo ye-assay ikhonaQedela, ulahla ama-specimens ngemuva kokuzivocavotela ngokuzenzakalela ku-121 ℃ okungenani20 imiz. Ngenye indlela, bangalashwa ngo-0.5% sodium hypochloriteamahora amaningi ngaphambi kokulahlwa.
■ Musa ukwenza i-pipette reagent ngomlomo futhi kungabhemi noma ukudla ngenkathi kwenziwaAMASO.
■ Gqoka amagilavu ​​phakathi nenqubo yonke.