Ukusetshenziswa okuhlosiwe
I-grontstep^®I-Adenovirus Rapid Test Test (FECES) ingukubukwa okusheshayoImmunoassway yokutholwa kokungezwani kwe-adenovirus kumuntuIzinhlobo ze-fecal. Le kit yenzelwe ukusetshenziswa njengosizo ekuhlolweni kwe-adenovirus
Ukutheleleka.

Ukuqalisa
Ama-adenoviruses angeziwe, ngokuyinhloko ad40 ne-AD41, ayimbangela ehamba phambili yesifo sohudoEzinganeni eziningi zihlushwa isifo sohudo esinamandla, sesibilikuma-rotaviruses kuphela. Isifo sokuhlanza i-acute haise iyimbangela enkulu yokufaEzinganeni ezincane emhlabeni wonke, ikakhulukazi emazweni asathuthuka. I-Adenovirusamagciwane ahlukaniswe emhlabeni wonke, futhi angadala isifo sohudoezinganeni unyaka nonyaka. Ukutheleleka kuvame ukubonwa kakhulu ezinganeni ezingaphansiEminyakeni emibili ubudala, kepha kutholakele ezigulini zeminyaka yonke.Ucwaningo lukhombisa ukuthi ama-adenoviruse ahlotshaniswa no-4-15% wabo bonkeAmacala angeniswe esibhedlela se-viral gastroenteritis.

Ukutholwa okusheshayo nokunembile kwe-adenovirus ehlobene ne-gastroenteritis kuyasizaEkusunguleni i-Etiology of Gastroenteritis kanye nokuphathwa kweziguli okuhlobene.Amanye amasu wokuxilonga afana ne-elektroni microscopy (em) futhiI-nucleic acid hybridization iyabiza futhi iyasebenza kanzima. KunikezweImvelo yokuzikhawulela yokutheleleka kwe-adenovirus, ebizayo futhiUkuhlolwa okunamandla abasebenzi kungenzeka kungadingeki.

Isimiso
I-Adenovirus Rapid Test Test (FECES) Ithola i-AdenovirusNgokuhumusha okubukwayo kokuthuthukiswa kombala kwangaphakathistrip. Ama-anti-adenovirus antibodies angafakwanga esifundeni sokuhlolwa kweulwelwesi. Ngesikhathi sokuhlola, okucatshangelwe ngama-anti-anti-adenovirus antibodiesKuhlanganiswe nezinhlayiya ezinemibala futhi zivezwe ephepheni lesampula lokuhlolwa.Ingxube isuka ku-membrane ngesenzo se-capillary futhi ixhumanangama-reagents ku-membrane. Uma kune-adenovirus eyanele ku-specimen, aIbhendi enemibala izokwakha esifundeni sokuhlola se-membrane. Ukuba khona kwalokhuIbhendi enemibala ikhombisa umphumela omuhle, kanti ukungabikho kwayo kukhombisa okubiumphumela. Ukubukeka kwebhendi enemibala eSifundeni Sokulawula kusebenza njengeUkulawulwa Kwenqubo, okubonisa ukuthi umthamo ofanele we-specimen ubelokhuokwengeziwe nokwenziwe ngoMembrane Wick kuye kwenzeka.

Indlela yokwenza izinto
Letha izivivinyo, ama-specimentens, buffer kanye / noma izilawuli zokushisa kwegumbi(15-30 ° C) ngaphambi kokusebenzisa.
1. Iqoqo le-specimen kanye nokwelashwa kwangaphambili:
1) Sebenzisa iziqukathi ezihlanzekile, ezomile zokuqoqwa kwe-specimen. Imiphumela emihle kakhulu izobaetholakele uma kutholakala i-assay kungakapheli amahora angu-6 aqoqiwe.
2) Ngemifanekiso eqinile: Susaw bese ususa ofakayo we-dilution tube. YibaQikelela ukuthi ungachithi noma isixazululo esisehlobe kusuka kushubhu. Qoqa ama-specimentensNgokufaka i-actificator anamathele okungenani amasayithi amathathu ahlukene weI-FECES ukuqoqa cishe i-50 mg ye-FECES (okulingana no-1/4 wethanga).Ngezinhlobo zamatshe awuketshezi: bamba i-pipete mpo, i-aspirate fecalama-splemens, bese udlulisa amaconsi ama-2 (cishe ama-80 μL) kuI-Specimen Collection Tube equkethe i-buffer yokukhipha.
3) Faka esikhundleni somfakisicelo emuva ku-tube bese usula i-cap ngokuqinile. YibaQikelela ukuthi ungaphuli isihloko se-dilution tube.
4) Shake ishubhu leqoqo le-specimen ngamandla ukuxubana okucacisiwe futhiisiphazamiso sokukhishwa. Izinhlobo ezilungiselelwe ku-specimen Collection tubeingagcinwa izinyanga eziyi-6 ku-20 ° C Uma kungahloliwe kungakapheli ihora eli-1Ukulungiselela.

2. Ukuhlola
1) Susa isivivinyo esikhwameni sayo esivalekile, bese uyibekaindawo ehlanzekile, yezinga. Faka ilebula isivivinyo ngesiguli noma sokulawulaukukhonjwa. Ngemiphumela emihle, i-assay kufanele yenziwe ngaphakathi kweyodwaihora.
2) Usebenzisa ucezu lwephepha lezicubu, phula ithiphu ye-dilution tube. BambaI-tube mpo futhi ukhiphe amaconsi ama-3 wesixazululo ku-specimen kahle(S) yedivayisi yokuhlola.Gwema ukudonsa ama-bubble air ku-specimen kahle (ama), futhi ungangezi
Noma yisiphi isixazululo sewindi lemiphumela.Njengoba isivivinyo siqala ukusebenza, umbala uzohamba ngaphesheya kwe-membrane.

3. Lindela amabhendi anemibala ukuze avele. Umphumela kufanele ufundwe ngo-10imizuzu. Ungahumushi umphumela ngemuva kwemizuzu engama-20.

Qaphela:Uma i-specimen ingahambi ngenxa yokuba khona kwezinhlayiya, i-centrifugeama-specimens akhishwe okuqukethwe ku-adriction buffer vial. Qoqa i-100 μl yeI-Supernatant, dlulisa ku-specimen kahle (s) yedivayisi entsha yokuhlola bese uqala futhi, kulandela imiyalo echazwe ngenhla.

Ukuqinisekiswa