UKUSETSHENZISWA OKUHLOSIWE
Isinyathelo Esiqinile^®Idivayisi ye-Adenovirus Rapid Test (Indle) iwukubuka okusheshayoI-immunoassay yokutholwa kwekhwalithi ye-adenovirus kumuntuizibonelo ze-fecal.Le kit ihloselwe ukusetshenziswa njengosizo ekuxilongweni kwe-adenovirus
ukutheleleka.

ISINGENISO
Ama-enteric adenoviruses, ikakhulukazi i-Ad40 kanye ne-Ad41, ayimbangela ehamba phambili yohudo.ezinganeni eziningi eziphethwe isifo sohudo esiyingozi, okwesibiliama-rotavirus kuphela.Isifo sohudo esiyingozi siyimbangela enkulu yokufaezinganeni ezincane emhlabeni wonke, ikakhulukazi emazweni asathuthuka.I-Adenovirusamagciwane asehlukanisiwe emhlabeni wonke, futhi angabangela isifo sohudoezinganeni unyaka wonke.Amagciwane avame ukubonakala ezinganeni ezingaphansiiminyaka emibili, kodwa itholakale ezigulini zabo bonke ubudala.Ucwaningo lubonisa ukuthi i-adenoviruses ihlotshaniswa ne-4-15% yazo zonkeamacala esibhedlela we-viral gastroenteritis.

Ukuxilongwa okusheshayo nokunembile kwe-adenovirus-related gastroenteritis kuyasizaekusunguleni i-etiology ye-gastroenteritis kanye nokuphathwa kwesiguli okuhlobene.Amanye amasu okuxilonga afana ne-electron microscopy (EM) kanyeI-nucleic acid hybridization iyabiza futhi idinga umsebenzi.KunikezweImvelo yokuzikhawulela yokutheleleka kwe-adenovirus, ebiza kakhulu futhiukuhlolwa okudinga abasebenzi kungase kungadingeki.

ISIMISO
I-Adenovirus Rapid Test Device (Indle) ithola i-adenovirusngokusebenzisa incazelo ebonakalayo yokuthuthukiswa kombala ngaphakathihlubula.Ama-anti-adenovirus amasosha omzimba awanyakazi endaweni yokuhlolwaulwelwesi.Ngesikhathi sokuhlolwa, isifanekiso siphendula ngama-anti-adenovirus amasosha omzimbakuhlanganiswe nezinhlayiya ezinemibala futhi kuhlanganiswe ngaphambili kuphedi yesampula yokuhlolwa.Ingxube ibe isihamba kulwelwesi ngesenzo se-capillary bese ixhumanangama-reagents kulwelwesi.Uma kune-adenovirus eyanele kusampula, aibhande elinemibala lizokwakheka endaweni yokuhlola yolwelwesi.Ukuba khona kwalokhuibhande elinemibala libonisa umphumela omuhle, kuyilapho ukungabikho kwawo kubonisa okungalungileumphumela.Ukubonakala kwebhande elinemibala endaweni yokulawula kusebenza njenge-aukulawulwa kwenqubo, okubonisa ukuthi umthamo ofanele we-specimen ubelokhuingeziwe futhi i-membrane wicking yenzekile.

INQUBO
Letha izivivinyo, izifanekiso, ibhafa kanye/noma izilawuli ekamelweni lokushisa(15-30 ° C) ngaphambi kokusetshenziswa.
1. Ukuqoqwa kwesampula nokwelashwa kwangaphambili:
1) Sebenzisa iziqukathi ezihlanzekile, ezomile ukuze uqoqe isifanekiso.Imiphumela emihle kakhulu izobakutholwe uma ukuhlolwa kwenziwa phakathi namahora angu-6 ngemva kokuqoqwa.
2) Ngezibonelo eziqinile: Khumula futhi ukhiphe isifakeli seshubhu lokuhlanjululwa.Yibaqaphela ukuthi ungachithi noma uthele isixazululo eshubhu.Qoqa izibonelongokufaka uthi lokufaka isicelo okungenani ezindaweni ezi-3 ezihlukene ze-indle yokuqoqa cishe u-50 mg wendle (okulingana ne-1/4 yephizi).Ukuze uthole izibonelo eziwuketshezi: Bamba i-pipette iqonde phezulu, i-aspirate fecalama-specimens, bese udlulisela amaconsi ama-2 (cishe ama-80 µL)ishubhu yokuqoqwa kwesifanekiso equkethe isilondolozi sokukhipha.
3) Faka esikhundleni isifakeli usibuyisele eshubhuni bese usikulufa ikepisi ngokuqinile.Yibaqaphela ukuthi ungaphuli ichopho le-dilution tube.
4) Shake ishubhu yokuqoqa isifanekiso ngamandla ukuze uhlanganise isifanekiso futhii-bafa yokukhipha.Izibonelo ezilungiselelwe kushubhu yokuqoqa isifanekisoingagcinwa izinyanga eziyisi-6 ku--20°C uma ingahlolwa phakathi nehora elingu-1 ngemva kwalokhoukulungiselela.

2. Ukuhlola
1) Khipha isivivinyo esikhwameni saso esivaliwe, bese usibekaindawo ehlanzekile, esezingeni.Lebula ukuhlolwa ngesiguli noma ngesilawuliukuhlonza.Ukuze uthole imiphumela engcono kakhulu, ukuhlolwa kufanele kwenziwe ngaphakathi kokukodwaihora.
2) Ukusebenzisa ucezu lwephepha lethishu, phula ichopho leshubhu lokuhlanjululwa.Bambaishubhu mpo bese ukhipha amaconsi angu-3 esixazululo emthonjeni wesampula(S) yedivayisi yokuhlola.Gwema ukuvalela amabhamuza omoya esibonelweni kahle (S), futhi ungangezi
noma yisiphi isisombululo sewindi lomphumela.Njengoba ukuhlola kuqala ukusebenza, umbala uzohamba udlulele kulwelwesi.

3. Linda kuze kuvele amabhendi anombala.Umphumela kufanele ufundwe ku-10imizuzu.Ungahumushi umphumela ngemuva kwemizuzu engama-20.

Qaphela:Uma i-specimen ingafuduki ngenxa yokuba khona kwezinhlayiya, i-centrifugeama-specimens akhishiwe aqukethwe ku-vial ye-baffer vial.Qoqa u-100 µL weamandla amakhulu, khipha esibonelweni somthombo (S) wensiza entsha yokuhlola bese uqala futhi, ulandela imiyalelo echazwe ngenhla.

Izitifiketi