Umhlaba owodwa impi eyodwa
─Ukubambisana kwamazwe ngamazwe ukwakha umphakathi womhlaba wonke wezephetho ezifanayo uphendula inselele yobhubhane lwe-COVID-19
I-coronavirus yenoveli eshanele emhlabeni wonke iholele enkingeni eqhubekayo yobhubhane lwe-COVID-19.I-coronavirus yenoveli ayinayo imingcele, alikho izwe elizosinda kule mpi yokulwa ne-COVID-19.Ukusabela kulo bhubhane lomhlaba wonke lwe-COVID-19, i-Liming Bio-Products Corp yenza iminikelo ukuze isekele inhlalakahle yemiphakathi yethu yomhlaba wonke.
Umhlaba wethu njengamanje ubhekene nomthelela ongakaze ubonwe wesifo esiwubhubhane se-coronavirus 2019 (COVID-19).Kuze kube manje, awukho umuthi osebenzayo otholakalayo wokwelapha lesi sifo.Kodwa-ke, izivivinyo eziningi zokuxilonga zenzelwe ukutholwa kwe-COVID-19.Lokhu kuhlola kusekelwe ezindleleni zamangqamuzana noma ze-serological zokuthola inoveli ye-nucleic acid ethile ye-coronavirus noma ama-antibody biomarker.Njengoba i-COVID-19 isifinyelele esimweni sobhubhane, ukutholakala kusenesikhathi kokutheleleka kwe-coronavirus yenoveli kubalulekile ekuhloleni ukusabalala kwaleli gciwane futhi liqukethe, kodwa ukuhlolwa okuphelele kokusetshenziswa kwendawo yonke akukakatholakali.Kufanele sazi ukuthi yiziphi izivivinyo ezingase zisetshenziselwe ukuhlola, ukuxilonga, nokuqapha ukutheleleka nge-COVID-19, nokuthi iyini imikhawulo yakho.Kubaluleke kakhulu ukuthi ungawasebenzisa kanjani kangcono la mathuluzi esayensi nokusiza ekuboneni nasekulawuleni ukuvela kwalesi sifo esibhebhetheka ngokushesha nesiyingozi.
Inhloso yokutholwa kwe-coronavirus yenoveli iwukuba kunqunywe ukuthi ngabe umuntu onaleli gciwane le-COVID-19 noma umthwali we-asymptotic ongase asakaze leli gciwane buthule, ukuze anikeze ulwazi olubalulekile lokuqondisa ukuthathwa kwezinqumo zokwelashwa.Ucwaningo lwangaphambilini lubonise ukuthi u-70% wezinqumo zomtholampilo zincike emiphumeleni yokuhlolwa.Lapho kusetshenziswa izindlela ezihlukene zokuhlonza, izimfuneko zamakhithi e-reagent yokuthola nazo zihlukile.
Umfanekiso 1
Umfanekiso1:Umdwebo obonisa izigaba ezibalulekile zamazinga ajwayelekile e-biomarker ngesikhathi esijwayelekile sokutheleleka nge-COVID-19.I-X-axis ikhombisa inani lezinsuku zokutheleleka, kanti i-Y-axis ikhombisa umthamo wegciwane egazini, ukugcwala kwama-antigen, kanye nokugcwala kwamasosha omzimba ngezikhathi ezahlukahlukene.I-antibody ibhekisela kumasosha omzimba e-IgM ne-IgG.Kokubili i-RT-PCR nokutholwa kwe-antigen kusetshenziselwa ukuthola ubukhona noma ukungabikho kwe-coronavirus yenoveli, okuwubufakazi obuqondile bokutholwa kwesiguli kusenesikhathi.Phakathi neviki lokutheleleka ngegciwane, ukutholwa kwe-PCR, noma ukutholwa kwe-antigen kuyakhethwa.Ngemuva kokutheleleka kwe-coronavirus yenoveli cishe izinsuku eziyi-7, i-antibody ye-IgM ngokumelene ne-coronavirus yenoveli ikhuphuke kancane kancane egazini lesiguli, kepha isikhathi sokuba khona sifushane, futhi ukugxila kwayo kuncipha ngokushesha.Ngokuphambene, i-antibody ye-IgG ngokumelene naleli gciwane ivela kamuva, imvamisa cishe izinsuku eziyi-14 ngemuva kokutheleleka ngegciwane.Ukuhlushwa kwe-IgG kancane kancane kuyanda, futhi kuqhubeka isikhathi eside egazini.Ngakho-ke, uma i-IgM itholwa egazini lesiguli, kusho ukuthi igciwane lisanda kungena, okuwuphawu lokutheleleka kwangaphambi kwesikhathi.Uma i-antibody ye-IgG itholwa egazini lesiguli, kusho ukuthi ukutheleleka ngegciwane sekunesikhathi eside.Kubizwa nangokuthi ukutheleleka sekwephuzile noma ukutheleleka kwangaphambilini.Kuvame ukubonakala ezigulini ezisesigabeni sokululama.
Ama-biomarker we-novel coronavirus
I-coronavirus yenoveli igciwane le-RNA, elakhiwe ngamaprotheni nama-nucleic acid.Igciwane lihlasela umzimba womsingathi (womuntu), lingene kumaseli lisebenzisa isamukeli esihambisana ne-ACE2 sesayithi elibophayo, futhi liphindaphindeke kumaseli asokhaya, okwenza amasosha omzimba womuntu aphendule kubahlaseli bangaphandle futhi akhiqize amasosha omzimba athile.Ngakho-ke, i-vial nucleic acids nama-antigens, kanye namasosha omzimba athile ngokumelene ne-coronavirus yenoveli angasetshenziswa njengezimpawu ezithile ze-biomarker zokutholwa kwe-coronavirus yenoveli.Ukuze kutholwe i-nucleic acid, ubuchwepheshe be-RT-PCR yibo obusetshenziswa kakhulu, kuyilapho izindlela ze-serological zivame ukusetshenziselwa ukuthola amasosha omzimba aqondene ne-coronavirus.Njengamanje, kunezindlela ezahlukahlukene zokuhlola ezitholakalayo esingazikhetha ukuze sihlole ukutheleleka nge-COVID-19 [1].
Izimiso eziyisisekelo zezindlela zokuhlola eziyinhloko ze-coronavirus yenoveli
Ukuhlola okuningi kokuxilonga kwe-COVID_19 kuyatholakala kuze kube manje, nezinsiza eziningi zokuhlola zithola ukugunyazwa ngaphansi kokugunyazwa kokusetshenziswa kwezimo eziphuthumayo nsuku zonke.Yize ukuthuthukiswa kokuhlolwa okusha kuphuma namagama namafomethi amaningi ahlukene, konke ukuhlolwa kwamanje kwe-COVID_19 ngokuyisisekelo kuncike kubuchwepheshe obubili obukhulu: ukutholwa kwe-nucleic acid ye-RNA yegciwane kanye nama-serological immunoassays athola amasosha omzimba aqondene negciwane (IgM ne-IgG).
01. Ukutholwa kwe-Nucleic acid
I-reverse transcription-polymerase chain reaction (RT-PCR), i-loop-mediated isothermal amplification (LAMP), kanye nokulandelanisa kwesizukulwane esilandelayo (NGS) izindlela ezivamile ze-nucleic acid zokutholwa kwenoveli i-coronavirus RNA.I-RT-PCR iwuhlobo lokuqala lokuhlolwa kwe-COVID-19, okunconywe yi-World Health Organization (WHO) kanye ne-US Center for Disease Control and Prevention (CDC).
02.Ukutholwa kwamasosha omzimba
I-antibody iyiphrotheni evikelayo ekhiqizwa emzimbeni womuntu ukuphendula ukutheleleka ngegciwane.I-IgM iwuhlobo lwakuqala lwe-antibody kuyilapho i-IgG iyi-antibody yohlobo lwakamuva.Isampula ye-serum noma ye-plasma imvamisa ihlolelwa ukuba khona kwezinhlobo ezithile ze-IgM ne-IgG ze-antibody ukuze kuhlolwe izigaba ezibucayi nezescent zokutheleleka nge-COVID-19.Lezi zindlela zokutholwa ezisekelwe kumasosha omzimba zihlanganisa i-colloidal gold immunochromatography assay, i-latex noma i-fluorescent microsphere immunochromatography, i-enzyme-linked immunosorbent assay (ELISA), kanye ne-chemiluminescence assay.
03.Ukutholwa kwe-antigen yegciwane
I-Antigen iyisakhiwo segciwane eliqashelwa ngumzimba womuntu esenza amasosha omzimba akhiqize amasosha omzimba ukuze asuse igciwane egazini nezicubu.I-antigen yegciwane ekhona kugciwane ingaqondiswa futhi ibonwe ngokusebenzisa i-immunoassay.Njenge-viral RNA, ama-antigen egciwane akhona futhi emgudwini wokuphefumula wabantu abanaleli gciwane futhi angasetshenziswa ukuhlonza isigaba esibucayi sokutheleleka kwe-COVID-19.Ngakho-ke, kuvame ukutuswa ukuba kuqoqwe izibonelo zokuphefumula okuphezulu njengamathe, ama-nasopharyngeal kanye ne-oropharyngeal swabs, isikhwehlela sokukhwehlela okujulile, uketshezi lwe-bronchoalveolar lavage(BALF) ukuze kuhlolwe i-antigen yokuqala.
Ukukhetha izindlela zokuhlola i-coronavirus entsha
Ukukhetha indlela yokuhlola kuhilela izici eziningi, okuhlanganisa ukulungiselelwa komtholampilo, ukulawulwa kwekhwalithi yokuhlola, isikhathi sokushintsha, izindleko zokuhlola, izindlela zokuqoqa amasampula, izidingo zobuchwepheshe zabasebenzi baselabhorethri, izidingo zesikhungo kanye nemishini.Ukutholwa kwama-nucleic acid noma ama-antigen egciwane kuwukunikeza ubufakazi obuqondile bokuba khona kwamagciwane futhi kuqinisekiswe ukutholakala kokutheleleka kwe-coronavirus yenoveli.Yize ziningi izindlela zokutholwa kwe-antigen, ukuzwela kwayo kokutholwa kwe-coronavirus yenoveli kuphansi ngokwethiyori kunaleyo yokukhulisa i-RT-PCR.Ukuhlola amasosha omzimba ukutholwa kwamasosha omzimba alwa namagciwane akhiqizwa emzimbeni womuntu, okusalibele ngesikhathi futhi awavamile ukusetshenziselwa ukutholwa kusenesikhathi phakathi nesigaba esibi kakhulu sokutheleleka ngegciwane.Isimo somtholampilo sezinhlelo zokusebenza zokuthola singahluka, futhi amasayithi amasampula amaqoqo nawo angahluka.Ukuze kutholwe i-viral nucleic acids nama-antigens, isifanekiso sidinga ukuqoqwa emgudwini wokuphefumula lapho igciwane likhona, njenge-nasopharyngeal swabs, i-oropharyngeal swabs, isikhwehlela, noma i-bronchoalveolar lavage fluid (BALF).Ukuze kutholwe amasosha omzimba, isifanekiso segazi sidinga ukuqoqwa futhi sihlolelwe ukuba khona kwe-anti-virus ethile (IgM/IgG).Kodwa-ke, imiphumela yokuhlolwa kwe-antibody ne-nucleic acid ingaphelelisana.Isibonelo, uma umphumela wokuhlolwa uthi i-nucleic acid-negative, i-IgM-negative kodwa i-IgG-positive, le miphumela ikhomba ukuthi isiguli okwamanje asinalo leli gciwane, kodwa sesitholakele ekuthelelekeni kwenoveli ye-coronavirus.[2]
Izinzuzo kanye nokubi kokuhlolwa kwe-coronavirus yenoveli
Ku-Diagnostic and Treatment Protocol for Novel Coronavirus Pneumonia (Trial Version7) (Ikhishwe yi-National Health Commission & State Administration of Traditional Chinese Medicine ngoMashi 3, 2020), ukuhlolwa kwe-nucleic acid kusetshenziswa njengendlela evamile yegolide yokuhlonza inoveli. ukutheleleka nge-coronavirus, kuyilapho ukuhlolwa kwe-antibody nakho kuthathwa njengenye yezindlela zokuqinisekisa zokuxilongwa.
Imiphumela ye-Pathogenic ne-serological
(1) Okutholwe yi-Pathogenic: I-Novel coronavirus nucleic acid ingatholwa ku-nasopharyngeal swabs, isikhwehlela, ukukhishwa kwepheshana lokuphefumula eliphansi, igazi, indle nezinye izibonelo kusetshenziswa izindlela ze-RT-PCRand/noma ze-NGS.Inembe kakhulu uma izibonelo zitholakala emgudwini wokuphefumula ophansi (isikhwehlela noma ukukhishwa kwepheshana lomoya).Izifanekiso kufanele zihanjiswe ukuze zihlolwe ngokushesha ngangokunokwenzeka ngemva kokuqoqwa.
(2) Okutholwe nge-Serological: I-NCP ethize ye-IgM yegciwane iqala ukutholakala cishe ezinsukwini ezi-3-5 ngemuva kokuqala;I-IgG ifinyelela ku-titration okungenani yokukhuphuka okuphindwe ka-4 ngesikhathi sokululama uma kuqhathaniswa nesigaba esibucayi.
Kodwa-ke, ukukhethwa kwezindlela zokuhlola kuncike ezindaweni, imithetho yezokwelapha, nezilungiselelo zomtholampilo.E-USA, i-NIH ikhiphe Iziqondiso Zokwelashwa Kwe-Coronavirus Disease 2019 (COVID-19) (Isizinda Esibuyekeziwe: Ephreli 21,2020 ) futhi i-FDA yakhipha Inqubomgomo Yokuhlolwa Kokuxilongwa Kwesifo Se-Coronavirus-2019 Ngesikhathi Sezimo Eziphuthumayo Zezempilo Yomphakathi (ikhishwe ngoMashi 16, 2020 ), lapho ukuhlolwa kwe-serological kwamasosha omzimba e-IgM/IgG kukhethwe kuphela njengokuhlolwa kokuhlola.
Indlela Yokuthola I-Nucleic Acid
I-RT_PCR ukuhlolwa okuzwela kakhulu kwe-nucleic acid okuklanyelwe ukuthola ukuthi i-RNA ye-coronavirus yenoveli ikhona yini emphefumulweni noma kwesinye isifanekiso.Umphumela wokuhlolwa one-PCR oqinisekile usho ukuba khona kwe-novel coronavirus RNA kusampula ukuze kuqinisekiswe ukutheleleka nge-COVID-19.Umphumela wokuhlolwa ongemuhle we-PCR awusho ukungabikho kokutheleleka ngegciwane ngoba kungase kuthinteke ikhwalithi yesampula ephansi noma iphuzu lesikhathi sesifo esigabeni esitholiwe, njll.Nakuba i-RT-PCR iwukuhlolwa okuzwela kakhulu, inezihibe ezimbalwa.Ukuhlolwa kwe-RT-PCR kungasebenza kanzima futhi kudle isikhathi, kuncike kakhulu kukhwalithi ephezulu yesampula.Lokhu kungaba inselele ngoba inani le-RNA yegciwane aligcini ngokuhluka kakhulu phakathi kweziguli ezihlukene kodwa futhi lingahluka phakathi kwesiguli esifanayo kuye ngamaphoyinti esikhathi lapho isampula liqoqwa khona kanye nezigaba zokutheleleka noma ukuqala kwezimpawu zomtholampilo.Ukuthola i-coronavirus yenoveli kudinga amasampula ekhwalithi ephezulu aqukethe inani elanele le-RNA yegciwane.
Ukuhlolwa kwe-RT-PCR kungase kunikeze umphumela omubi ongalungile (unegethivu yamanga) kwezinye iziguli ezinokutheleleka nge-COVID-19.Njengoba sazi, izindawo zokutheleleka eziphambili ze-coronavirus yenoveli zitholakala emaphashini nasemgudwini wokuphefumula ophansi, njenge-alveoli ne-bronchi.Ngakho-ke, isampula yesikhwehlela esivela ekukhwehleleni okujulile noma i-bronchoalveolar lavage fluid (BALF) ibhekwa njengokuzwela okuphezulu kakhulu kokutholwa kwegciwane.Kodwa-ke, ekusebenzeni komtholampilo, amasampula avame ukuqoqwa emgudwini wokuphefumula ophezulu ngokusebenzisa i-nasopharyngeal noma i-oropharyngeal swabs.Ukuqoqa lezi zifanekiso akugcini nje ngokunethezeka ezigulini kodwa futhi kudinga abasebenzi abaqeqeshwe ngokukhethekile.Ukwenza amasampula angangeni noma abe lula, kwezinye izimo iziguli zingase zinikezwe i-oral swab futhi zizivumele ukuthi zithathe isampula ku-buccal mucosa noma ukuziswahla ngolimi.Ngaphandle kwe-RNA yegciwane eyanele, i-RT-qPCR ingabuyisela umphumela wokuhlolwa wokuthi awunayo amanga.Esifundazweni sase-Hubei, e-China, ukuzwela kwe-RT-PCR ekutholweni kokuqala kubikwe kuphela mayelana nama-30% -50%, ngesilinganiso esingu-40%.Izinga eliphezulu lokuqamba amanga-negative cishe libangelwe ukungathathwa kwamasampuli okwanele.
Ngaphezu kwalokho, ukuhlolwa kwe-RT-PCR kudinga abasebenzi abaqeqeshwe kakhulu ukuze benze izinyathelo eziyinkimbinkimbi zokukhipha i-RNA kanye nenqubo yokukhulisa i-PCR.Kudingeka futhi izinga eliphezulu lokuvikelwa kwe-biosafety, indawo yaselabhorethri ekhethekile, kanye nethuluzi le-PCR lesikhathi sangempela.E-China, ukuhlolwa kwe-RT-PCR kokutholwa kwe-COVID-19 kudinga ukwenziwa kulabhorethri yezinga lesi-2 le-biosafety (BSL-2), ngokuvikelwa kwezisebenzi kusetshenziswa umkhuba we-biosafety level 3 (BSL-3).Ngaphansi kwalezi zidingo, kusukela ekuqaleni kukaJanuwari kuya ekuqaleni kukaFebhuwari 2020, amandla elabhorethri ye-CDC yaseChina Wuhan akwazile ukuthola amacala angamakhulu ambalwa ngosuku.Ngokuvamile, lokhu ngeke kube yinkinga lapho kuhlolwa ezinye izifo ezithathelwanayo.Kodwa-ke, lapho ubhekene nobhubhane lomhlaba wonke olufana ne-COVID-19 okungenzeka luhlolwe izigidi zabantu, i-RT-PCR iba yinkinga ebucayi ngenxa yezidingo zayo zezindawo ezikhethekile zaselabhorethri noma izinto zobuchwepheshe.Lokhu kubi kungase kukhawulele i-RT-PCR ukuthi isetshenziswe njengethuluzi elisebenzayo lokuhlola, futhi ingase iholele ekubambezelekeni kwemibiko yemiphumela yokuhlola.
Indlela yokuthola amasosha omzimba
Ngokuqhubeka kwesifundo sesifo, ikakhulukazi esigabeni esimaphakathi nesemuva, izinga lokutholwa kwamasosha omzimba liphezulu kakhulu.Ucwaningo olwenziwe eWuhan Central South Hospital luveze ukuthi izinga lokutholwa kwe-antibody lingafinyelela ngaphezu kwama-90% evikini lesithathu lokutheleleka nge-COVID-19.Futhi, i-antibody ingumkhiqizo wokusabela komzimba womuntu ngokumelene ne-coronavirus yenoveli.Ukuhlolwa kwe-antibody kunikeza izinzuzo ezimbalwa kune-RT-PCR.Okokuqala, i-serological antibody ihlola kalula futhi ngokushesha.Ukuhlolwa kokugeleza kwe-antibody lateral kungasetshenziselwa indawo yokunakekela ukuletha umphumela emizuzwini eyi-15.Okwesibili, okuhlosiwe okutholwe ukuhlolwa kwe-serological i-antibody, eyaziwa njengezinzile kakhulu kune-RNA yegciwane.Ngesikhathi sokuqoqwa, ukuthuthwa, ukugcinwa nokuhlolwa, izibonelo zokuhlolwa kwe-antibody ngokuvamile zizinzile kunezibonelo ze-RT-PCR.Okwesithathu, ngenxa yokuthi i-antibody isatshalaliswa ngokulinganayo ekujikelezeni kwegazi, kukhona ukuhluka okuncane kwamasampula uma kuqhathaniswa nokuhlolwa kwe-nucleic acid.Ivolumu yesampula edingekayo ekuhlolweni kwe-antibody incane uma kuqhathaniswa.Isibonelo, i-microliter engu-10 yegazi lokuhlaba umunwe yanele ukusetshenziswa ekuhlolweni kokugeleza kwe-antibody lateral.
Ngokuvamile, ukuhlolwa kwe-antibody kukhethwa njengethuluzi elingeziwe lokutholwa kwe-nucleic acid ukuze kuthuthukiswe izinga lokutholwa kwe-coronavirus yenoveli phakathi nezifundo zesifo.Uma ukuhlolwa kwe-antibody kusetshenziswa kanye nokuhlolwa kwe-nucleic acid, kungase kukhuphule ukunemba kokuhlolwa ukuze kutholwe i-COVID19 ngokunciphisa imiphumela engase ibe mihle kanye nemibi engamanga.Umhlahlandlela wamanje wokusebenza awuncomi ukusebenzisa izinhlobo ezimbili zokuhlola ngokuhlukene njengefomethi yokuthola ezimele kodwa kufanele isetshenziswe njengefomethi ehlanganisiwe.[2]
Umfanekiso 2:Ukuchazwa okulungile kwe-nucleic acid kanye nemiphumela yokuhlolwa kwe-antibody ukuze kutholwe ukutheleleka kwe-coronavirus yenoveli
Umfanekiso 3:I-Liming Bio-Products Co., Ltd. - Ikhithi yokuhlola esheshayo ye-Novel coronavirus IgM/IgG (StrongStep®Ukuhlolwa Okusheshayo kwe-SARS-CoV-2 IgM/IgG Antibody, i-Latex Immunochromatography)
Umfanekiso 4:I-Liming Bio-Products Co., Ltd. - StrongStep®Ikhithi ye-PCR ye-Novel Coronavirus (SARS-CoV-2) Multiplex Real-Time PCR (ukutholwa kwezakhi zofuzo ezintathu, indlela ye-fluorescent probe).
Qaphela:Le kit ye-PCR ezwela kakhulu, elungele ukusetshenziswa itholakala ngefomethi ye-lyophilized (inqubo yokumisa iqhwa) ukuze igcinwe isikhathi eside.Ikhithi ingathuthwa futhi igcinwe ekamelweni lokushisa futhi izinzile unyaka owodwa.Ishubhu ngalinye le-premix liqukethe zonke izixube ezidingekayo ekukhuliseni i-PCR, okuhlanganisa i-Reverse-transcriptase, i-Taq polymerase, ama-primer, ama-probes, nama-substrates e-dNTPs. ethuluzini le-PCR ukuze usebenzise ukukhulisa.
Ukusabela ekuqubukeni kwenoveli ye-coronavirus, i-Liming Bio-Products Co., Ltd. isebenze ngokushesha ukuze yakhe izinsiza zokuxilonga ezimbili ukuze inikeze amandla izindawo zokucwaninga zezempilo zomphakathi nezempilo ukuthi zihlonze ngokushesha ukutheleleka nge-COVID-19.Lawa makhithi alungele ukusetshenziswa ekuhlolweni okukhulu emazweni nasezifundeni lapho ukuqubuka kwe-coronavirus yenoveli kubhebhetheka khona ngokushesha, kanye nokuhlinzeka ngokuxilongwa nokuqinisekisa ukutheleleka nge-COVID-19.Lawa makhithi awokusetshenziswa kuphela ngaphansi Kokugunyazwa Kokusetshenziswa Kwezimo Eziphuthumayo (PEUA).Ukuhlola kukhawulelwe kumalabhorethri aqinisekiswe ngaphansi kwemithetho yeziphathimandla zikazwelonke noma zendawo.
Indlela yokuthola i-antigen
1. Ukutholwa kwe-antigen yegciwane kuhlukaniswa esigabeni esifanayo sokutholwa okuqondile njengokutholwa kwe-nucleic acid.Lezi zindlela zokuhlonza ngokuqondile zibheka ubufakazi bezifo ezibangelwa amagciwane esifanekisweni futhi zingasetshenziselwa ukuqinisekiswa kokuxilongwa.Kodwa-ke, ukuthuthukiswa kwamakhithi okuthola ama-antigen kudinga izinga eliphezulu lama-antibodies e-monoclonal anobudlelwane obuqinile nokuzwela okuphezulu okukwazi ukubona nokubamba amagciwane e-pathogenic.Ngokuvamile kuthatha isikhathi esingaphezu kwezinyanga eziyisithupha ukukhetha kanye nokuthuthukisa i-monoclonal antibody efanelekile ukuze isetshenziswe ekulungiseni ikhithi yokuthola i-antigen.
2. Njengamanje, ama-reagents okutholwa ngokuqondile kwe-coronavirus yenoveli asengaphansi kwesigaba socwaningo nentuthuko.Ngakho-ke, alikho ikhithi yokuthola i-antigen eye yaqinisekiswa ngokomtholampilo futhi yatholakala ngokwentengiso.Nakuba ngaphambili kwabikwa ukuthi inkampani yokuxilonga e-Shenzhen ithuthukise ikhithi yokuthola i-antigen futhi yahlolwa ngomtholampilo e-Spain, ukwethembeka kokuhlolwa nokunemba akukwazanga ukuqinisekiswa ngenxa yokuba khona kwezinkinga zekhwalithi ye-reagent.Kuze kube manje, i-NMPA (yangaphambili yase-China FDA) ayikagunyazi noma iyiphi ikhithi yokuthola i-antigen ukuze isetshenziswe emtholampilo okwamanje.Sengiphetha, kuye kwenziwa izinhlobonhlobo zezindlela zokuhlonza.Indlela ngayinye inezinzuzo zayo nokulinganiselwa kwayo.Imiphumela evela ezindleleni ezahlukene ingasetshenziswa ukuze kuqinisekiswe futhi kupheleliswe.
3. Ukukhiqiza ikhithi yokuhlola yekhwalithi ye-COVID-19 kuncike kakhulu ekuthuthukisweni phakathi kocwaningo nentuthuko.I-Liming Bio-Product Co., Ltd.amakhithi okuhlola ayadingeka ukuze ahlangabezane nezindinganiso eziqinile zokukhiqiza kanye nokulawula ikhwalithi ukuze kuqinisekiswe ukuthi ahlinzeka ngamazinga aphezulu kakhulu okusebenza nokuvumelana.Ososayensi base-Liming Bio-Product Co., Ltd. banokuhlangenwe nakho kweminyaka engaphezu kwamashumi amabili ekuklameni, ekuhloleni, nasekuthuthukiseni amakhithi okuxilonga e-in vitro ukuze kuqinisekiswe izinga eliphezulu lokusebenza ekubalweni kokuhlaziya.
Ngesikhathi se-COVID-19 Pandemic, uhulumeni waseChina ubhekane nesidingo esikhulu sezinto zokuvikela ubhubhane ezindaweni ezishisayo zamazwe omhlaba.Ngomhla zi-5 kuMbasa, engqungqutheleni yabezindaba ye-State Council Joint Prevention and Control Mechanism "Ukuqinisa Ukuphathwa Kwekhwalithi Yempahla Yezokwelapha kanye Nokulawula Ukuhleleka Kwemakethe", uJiang Fan, umhloli wezinga lokuqala woMnyango Wezohwebo Zangaphandle Womnyango of Commerce, uthe, "Okulandelayo, sizogxila emizamweni yethu ezicini ezimbili, okokuqala, ukusheshisa ukusekelwa kwempahla yezokwelapha eyengeziwe edingwa umphakathi wamazwe ngamazwe, futhi, ukuthuthukisa ukulawulwa kwekhwalithi, ukulawulwa, nokuphathwa kwemikhiqizo. Sizokwenza umnikelo weShayina ekuphenduleni ngokuhlanganyela ubhubhane lomhlaba wonke futhi sakhe umphakathi onekusasa elihlanganyelwe lesintu.
Umfanekiso 5:Inkampani ye-Liming Bio-Products Co., Ltd. yenoveli ye-coronavirus ithole isitifiketi sokubhaliswa kwe-EU CE
Isitifiketi sokuhlonishwa
Houshenshan
Umfanekiso 6. I-Liming Bio-Products Co., Ltd. isekele i-Wuhan Vulcan(HouShenShan) Mountain Hospital ukuze silwe nobhubhane lwe-COVID-19 futhi yaklonyeliswa ngesitifiketi sokuhlonishwa se-Wuhan Red Cross.I-Wuhan Vulcan Mountain Hospital yisibhedlela esidume kakhulu e-China esisebenza ngokukhethekile ekwelapheni iziguli ezinzima ze-COVID-19.
Njengoba ukuqubuka kwe-coronavirus yenoveli kuqhubeka nokusabalala emhlabeni wonke, i-Nanjing Liming Bio-Products Co., Ltd. Ikhuphuka ukuze isekele futhi isize imiphakathi emhlabeni wonke ngobuchwepheshe bethu obusha bokulwa nalolu songo lomhlaba wonke olungakaze lubonwe.Ukuhlolwa okusheshayo kokutheleleka nge-COVID-19 kuyingxenye ebalulekile yokubhekana nalolu songo.Siyaqhubeka nokufaka isandla ngendlela ephawulekayo ngokuhlinzeka ngezinkundla zokuxilonga ezisezingeni eliphezulu ezandleni zabasebenzi bezempilo abaphambili ukuze abantu bathole imiphumela yokuhlolwa ebucayi abayidingayo.Imizamo ye-Liming Bio-products Co., Ltd. ekulweni nobhadane lwe-COVID-19 iwukuba sinikele ngobuchwepheshe bethu, ulwazi lwethu, nolwazi lwethu emiphakathini yamazwe ngamazwe ukuze kwakhiwe umphakathi womhlaba wonke wekusasa.
Cindezela Okude~Skena futhi Usilandele
I-imeyili: sales@limingbio.com
Iwebhusayithi: https://limingbio.com
Isikhathi sokuthumela: May-01-2020
