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-Izizukulwane ze-SARS-Cov-2 Ukuxilongwa kokutheleleka
Kwamacala aqinisekisiwe we-Covid-19 amacala, abike izimpawu ezijwayelekile zomtholampilo zifaka umkhuhlane, ukukhwehlela, i-myalgia noma ukukhathala. Kodwa-ke lezi zimpawu akuyona izici ezihlukile ze-Covid-19 ngoba lezi zimpawu zifana nalezo zesifo esinegciwane elinegciwane elifana nomkhuhlane. Njengamanje, i-virus nucleic acid real-time PCR (RT-PCR), ukucabanga kwe-CT namanye amapharamitha we-hematolology ngamathuluzi aphambili wokuxilongwa komtholampilo. Ama-elebhu amaningi welebhu athuthukisiwe futhi asetshenziswa ekuhlolweni kwezinhlobo zeziguli ze-Covid-19 nge-China CDC1, US CDC2nezinye izinkampani ezizimele. I-IGG / IGM Antibody Test, Indlela Yokuhlola Yezimali, nayo ingezwe njengenqubo yokuxilonga enguqulo evuselelwe eChina yokuxilongwa kanye nemihlahlandlela yokwelashwa yesifo senoveli ye-coronavirus (COVID-19), eyakhishwa ngomhlaka 31. I-virus nucleic acid RT-PRC Iyindlela yamanje yokuxilonga ejwayelekile yokuxilongwa kwe-Covid-19.
I-strongstep®Inoveli coronavlrus (SARS-Cov-2) I-Multiplex Real-Time PCR KIT (ukutholwa kwezinhlobo ezintathu)
Kodwa-ke lezi zinto zokuhlola ze-PCR zangempela ze-PCR, zifuna izinto zofuzo zaleli gciwane, ngokwesibonelo kuma-nasal, ngomlomo, noma ama-anal swabs, ahlushwa ukulinganiselwa okuningi:
1) Lezi zivivinyo zinezikhathi ezinde eziguqukayo futhi ziyinkimbinkimbi ekusebenzeni; Ngokuvamile bathatha ngokwesilinganiso amahora angaphezu kuka-2 kuye kwangama-3 ukukhiqiza imiphumela.
2) Ukuhlolwa kwe-PCR kudinga ama-laboratories aqinisekisiwe, imishini ebizayo kanye nochwepheshe abaqeqeshiwe ukuze basebenze.
3) Kunezinombolo ezithile zezinto ezimbi zamanga ze-RT-PCR ye-Covil-19 ye-19. Kungenzeka ngenxa yomthwalo wegciwane ophansi-1 wasula leli gciwane emizimbeni yabo.
Ucwaningo nguLirong Zou et al4uthole ukuthi imithwalo yegciwane ephakeme itholwe kungekudala ngemuva kokuqala kophawu, okutholakale imithwalo yegciwane ephakeme ekhaleni kunomphimbo kanye ne-viral nucleic acid specing of angenwe yi-SARS-Cov-2 ifana neziguli ezinomkhuhlane4Futhi kuvela okuhlukile kulokho okubonwe ezigulini ezingenwe yi-SARS-Cov-2.
Yang pan et al5kuhlolwe amasampula e-serial (Sputum, umchamo kanye nesitulo) kusuka ezigulini ezimbili eBeijing futhi wathola ukuthi amasampula e-viral aqala ukugcwala, amasampula we-sputum ngokuvamile akhombisa imithwalo yegciwane ephezulu kunalokho Amasampula we-Throat Swab. Alikho i-RNA ye-viral etholwe emchameni noma kumasampula ezihlahleni ezivela kulezi ziguli ezimbili.
Ukuhlolwa kwe-PCR kuphela kunikeza umphumela omuhle lapho leli gciwane selisekhona. Ukuhlolwa akunakukhomba abantu abadlula ekuthelelekeni, batholakele, basula leli gciwane emizimbeni yabo. Ngaphansi, cishe ama-30% -50% abehle ku-PCR ezigulini ezinonoveli we-coronavirus pneumonia. Iziguli eziningi zenoveli ye-coronavirus pneumonia azitholakalanga ngenxa yokuhlolwa okungalungile kwe-nucleic acid, ngakho-ke ngeke bakwazi ukuthola ukwelashwa okuhambisanayo ngesikhathi. Kusuka kokuqala kuya kohlelo lwesithupha lwezinkombandlela, ukuncika kuphela kusisekelo sokuxilongwa kwemiphumela yokuhlolwa kwe-nucleic acid, okwabangela inkathazo enkulu etholakawini. Isibhedlela, sifile. Ngesikhathi sokuphila kwakhe, wayenovivinyo oluntathu lwe-nucleic acid esimweni somkhuhlane nokukhwehlela, futhi okokugcina lapho ethola imiphumela emihle ye-PCR.
Ngemuva kwengxoxo ngochwepheshe, kwanqunywa ukukhulisa izindlela zokuhlola ama-serum njengendlela entsha yokuxilonga. Ngenkathi izivivinyo ze-antibody, ezibizwa nangokuthi izivivinyo ze-terological, ezingakuqinisekisa ukuthi othile ungenwe yize ngemuva kokuba amasosha abo omzimba aqede leli gciwane elibangela i-aveli-19.
I-GrostStep® SARS-Cov-2 iGG / IGM Antibody Rapid Test
I-IGG / IGM Antibody Test izosiza ekulandeleleni ngendlela esekwe kaninginingi ebiye nokutheleleka, ngoba amacala amaningi abonakala esatshalaliswa ezivela ezigulini ze-asymptomatic ezingakwazi ukutholakala kalula. Umbhangqwana eSingapore, umyeni wahlolelwa ukuthi une-PCR, umphumela wokuhlolwa komkakhe we-PCC's wawungemihle, kodwa imiphumela yokuhlolwa kwe-antibody yabonisa ukuthi wayenama-antibodies, njengoba kwenza umyeni wakhe.
I-Serological Assay idinga ukuqinisekiswa ngokucophelela ukuze uqiniseke ukuthi bayasabela ngokwethembekile, kodwa kuphela kuma-antibodies amelana negciwane leveli. Okunye ukukhathazeka kwakuwukuthi ukufana phakathi kwamagciwane adala isifo esiphezulu sokuphefumula okunamandla ne-19 (19 kungaholela ekuqhubekeni nasekusebenzeni. I-IGG-IGM yathuthukiswa yi-xue feng wang6bekubhekwa ukuthi akwazi ukusetshenziswa njengovivinyo lokunakekelwa kwamaphoyinti (i-POCT), njengoba kungenzeka kwenziwa eduze kombhede ngegazi. I-kit inokuzwela kwama-88.66% nokucaciswa kwama-90.63%. Kodwa-ke, kwase kunemiphumela emibi ebi namanga.
Enguqulo ebuyekeziwe yaseChina yokuxilongwa kanye nemihlahlandlela yokwelashwa yesifo se-Novel Coronavirus (Covid-19)1, amacala aqinisekisiwe achazwa njengamacala asolwayo ahlangabezana nanoma iyiphi enye yale mibandela elandelayo:
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Ukuxilongwa nokwelashwa kwe-Covid-19
| Imihlahlandlela | Ebilisiwe | Inqubo eqinisekisiwe yokuxilonga |
| Uhlobo 7th | 3mar.2020 | ❶ pcr ❷ ngs ❸ Igm + Igg |
| Inguqulo 6th | 18 Feb.2020 | ❶ pcr ❷ ngs |
Inkomba
1. Imihlahlandlela yokuxilongwa kanye nokwelashwa kwe-Novel Coronavirus Pneumonia (Uhlobo lweSivivinyo 7, IKhomishini Yezempilo Kazwelonke yeRepublic yabantu baseChina, ikhishwe ku-3.Mar.2020)
http://www.nhc.gov.cn/yzygj/s7652m/202003/311191442E29474b98bfed5549d5AF95.shtml
2
Norps.gov/coronavirus/2019-ncov/lab/rt-ccrouction-crmctractictions.html
3. I-Singapore ithi ukusetshenziswa kokuqala kokuhlolwa kwe-antibody ukulandelela ukutheleleka kwama-coronavirus
https:/www.sciensemag.org/news/2020/02/singapore-claimis-first-use-antibody-test-track-coronavirus-inthection
4.Sars-Cov-2-Viral Viral Izinhlobo zezilwane eziphakeme zokuphefumula kweziguli ezithelelekile ngoFebhuwari 19,2020 DoI: 10.1056 / NeJCC2001737
I-5.Viralloads ye-SARS-COV-2 kumasampula emtholampilo Lancet aqiniswe nge-inthanethi ngo-2020 eshicilelwe online ngoFebhuwari 24, 2020 (https:/10.16/s1473-3099-4)
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Ukuxilongwa kokutheleleka kwe-Xuefeng Wang Orcid ID: 0000-0001-8854-275X
Isikhathi sePosi: Mar-17-2020
